Phospho-RPS6KA1(T359) Antibody

SKU: orb1931132

Description

Research Area

Cell Biology, Immunology & Inflammation, Signal Transduction

Images & Validation

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Item 1 of 2

Tested Applications	DOT, WB
Dilution Range	WB - 1:2000, DB - 1:500
Reactivity	Human
Predicted Reactivity	Gallus, Xenopus

Key Properties

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Host	Rabbit
Clonality	Polyclonal
Isotype	Rabbit IgG
Molecular Weight	82723 Da
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
Form/Appearance	Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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MAPKAPK1A, RSK1

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Dot blot analysis of anti-RPS6KA1-pT359 Pab on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.5ug per ml.

Western blot analysis of extracts from Hela cells, untreated or treated with TPA, 200nM, using phospho RPS6KA1-T359 (left) or RPS6KA1 antibody (right)

Quick Database Links

UniProt

Q15418

UniProt Details

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No UniProt data available

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Protocol Information

Western Blot (IB, immunoblot)

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DOT

Dot Blot

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