Phospho-Stat1 (Ser727) (C6) rabbit mAb Antibody

SKU: orb1946146

Description

Stat1 mediates the cellular response to IFNŒ±, IFNB, and IFNg for the regulation of cell growth and the defense against viral and immune challenges. The Jak-Stat pathway plays a central role in the IFNg response, where Stat1 phosphorylation on Tyr701 causes homodimerization through its SH2 domain, translocation to the nucleus, and binding to gamma-activated sequence (GAS) elements. Early in the activation sequence, Stat1 is also phosphorylated at Ser727 through a mechanism involving PI3 kinase and Akt. Stat1 has been found to correlate with increased resistance to chemotherapeutic drugs. However, Stat1 activation of the immune system helps suppress tumor growth, and multiple melanomas and squamous-cell carcinomas have been known to downregulate Stat1 expression to evade immune surveillance.

Images & Validation

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Tested Applications	FC, WB
Dilution range	1µg/mL - 0.001µg/mL. It is recommended that the reagent be titrated for optimal performance for each application. See product image legends for additional information.
Reactivity	Human

Key Properties

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Host	Rabbit
Clonality	Monoclonal
Isotype	Rabbit IgG1k
Clone No.	Stat1S727-C6
Immunogen	A synthetic phospho-peptide corresponding to residues surrounding Ser727 of human phospho Stat1
Purification	Protein A+G
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/Appearance	Liquid
Buffer/Preservatives	1X PBS, 0.02% NaN3, 50% Glycerol, 0.1% rAlbumin
Concentration	0.5 mg/mL
Disclaimer	For research use only

Alternative Names

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Signal transducer and activator of transcription 1-alpha/beta, Transcription factor ISGF-3 components p91/p84

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Phospho-Stat1 (Ser727) (C6) rabbit mAb Antibody

Flow cytometric analysis of U937 cells untreated (red) or treated with IFNa + IL4 + PV (green) using Phospho-Stat1 (Ser727) (C6) Rabbit mAb Stat1S727-C6 at 0.01 ug/mL #orb1946146, or concentration-matched Rabbit (G9) mAb IgG Isotype Control #orb1946241 for cells untreated (black) or treated with IFNa + IL4 + PV (blue).

Western blot analysis of U937 cell extract untreated or treated with IL-4 using 0.1 μg/mL Phospho-Stat1 (Ser727) antibody Stat1S727-C6 Cat. #orb1946146.

Western blot analysis of U937 cell extract untreated or treated with sorbitol using 0.005 μg/mL Phospho-Stat1 (Ser727) antibody Stat1S727-C6 Cat. #orb1946146 or Company C antibody at 0.04 μg/mL (manufacturer's recommended concentration) developed using the same exposure.

Quick Database Links

UniProt

P42224

UniProt Details

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No UniProt data available

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Protocol Information

Western Blot (IB, immunoblot)

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Flow Cytometry

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