Phospho-Stat1 (Tyr701) (3E6) rabbit mAb Antibody

SKU: orb1946188

Description

Stat1 mediates the cellular response to IFNŒ±, IFNB, and IFNg for the regulation of cell growth and the defense against viral and immune challenges. The Jak-Stat pathway plays a central role in the IFNg response, where Stat1 phosphorylation on Tyr701 causes homodimerization through its SH2 domain, translocation to the nucleus, and binding to gamma-activated sequence (GAS) elements. Early in the activation sequence, Stat1 is also phosphorylated at Ser727 through a mechanism involving PI3 kinase and Akt. Stat1 has been found to correlate with increased resistance to chemotherapeutic drugs. However, Stat1 activation of the immune system helps suppress tumor growth, and multiple melanomas and squamous-cell carcinomas have been known to downregulate Stat1 expression to evade immune surveillance.

Images & Validation

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Item 1 of 4

Tested Applications	FC
Dilution range	1µg/mL - 0.001µg/mL. It is recommended that the reagent be titrated for optimal performance for each application. See product image legends for additional information.
Reactivity	Human, Mouse

Key Properties

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Host	Rabbit
Clonality	Monoclonal
Isotype	Rabbit IgG1k
Clone No.	Stat1Y701-3E6
Immunogen	A synthetic phospho-peptide corresponding to residues surrounding Tyr701 of human phospho Stat1
Purification	Protein A+G
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/Appearance	Liquid
Buffer/Preservatives	1X PBS, 0.02% NaN3, 50% Glycerol, 0.1% rAlbumin
Concentration	0.5 mg/mL
Disclaimer	For research use only

Alternative Names

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Signal transducer and activator of transcription 1-alpha/beta, Transcription factor ISGF-3 components p91/p84

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Phospho-Stat1 (Tyr701) (3E6) rabbit mAb Antibody

Peptide blocking flow cytometric analysis of U937 cells secondary antibody only negative control (light blue) or untreated (red) or treated with IFNα IL-4 and pervanadate (green) or untreated and blocked with phospho-peptide (black) or treated and blocked with phospho peptide (gold) or untreated and blocked with non-phospho peptide (dark blue) or treated and blocked with non-phospho peptide (purple) using Phospho-Stat1 (Tyr701) antibody Stat1Y701-3E6 at 0.1μg/mL. Cat. #orb1946188.

Flow cytometric analysis of NIH3T3 cells secondary antibody only negative control (blue) or 0.1 μg/mL of isotype control Cat. #orb1946241 (orange) or treated with imatinib (red) or with pervanadate (green) using Phospho-Stat1 (Tyr701) antibody Stat1Y701-3E6 at 0.1 μg/mL. Cat #orb1946188.

Flow cytometric analysis of U937 cells secondary antibody only negative control (blue) or untreated (red) or treated with IFNα IL-4 and pervanadate (green) using Phospho-Stat1 (Tyr701) antibody Stat1Y701-3E6 at 0.005 μg/mL Cat. #orb1946188.

Flow cytometric analysis of U937 cells secondary antibody only negative control (blue) or untreated (red) or treated with IFNα + IL-4 + pervanadate (green) using 0.1 ug/mL of Phospho-Stat1 (Tyr701) antibody Stat1Y701-3E6 Cat. #orb1946188 or Company C antibody at 0.2 ug/mL (manufacturer's recommended concentration).

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UniProt

P42224

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No UniProt data available

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Protocol Information

Flow Cytometry

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