Phospho-Stat6 (Tyr641) (G12) rabbit mAb Antibody

SKU: orb1946356

Description

The transcription factor Stat6 is a member of the signal transducers and activators of transcription (STAT) family of proteins. Stat6 is the only member of this family that is activated by interleukin-4 (IL-4), after which Stat6 is both tyrosine- and serine-phosphorylated by Jak kinases. The consensus Stat6 binding site TTCN4GAA is found in the promoters of many genes regulated by IL-4. In T lymphocytes, Stat6 is required for differentiation into Th2 cells in response to IL-4. Stat6 may play a role in solid tumorigenesis; a large immunohistochemistry study of Stat6 expression in over 2,000 tumor samples confirmed strong nuclear staining.

Images & Validation

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Item 1 of 5

Tested Applications	FC, WB
Dilution Range	1µg/mL - 0.001µg/mL. It is recommended that the reagent be titrated for optimal performance for each application. See product image legends for additional information.
Reactivity	Human, Mouse

Key Properties

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Host	Rabbit
Clonality	Monoclonal
Isotype	Rabbit IgG1k
Clone No.	Stat6Y641-G12
Immunogen	A synthetic phospho-peptide corresponding to residues surrounding Tyr641 of human phospho Stat6
Purification	Protein A+G
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/Appearance	Liquid
Buffer/Preservatives	1X PBS, 0.02% NaN3, 50% Glycerol, 0.1% rAlbumin
Concentration	0.5 mg/mL
Disclaimer	For research use only

Alternative Names

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Signal transducer and activator of transcription 6, IL-4 Stat

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Phospho-Stat6 (Tyr641) (G12) rabbit mAb Antibody

Western blot analysis of Daudi cell extract untreated or treated with IL-4 using 0.01μg/mL Phospho-Stat6 (Tyr641) antibody Stat6Y641-G12. Cat. #orb1946356.

Flow cytometric analysis of NIH3T3 cells secondary antibody only negative control (blue) or 0.1 μg/mL of isotype control Cat. #orb1946241 (orange) or treated with imatinib (red) or with pervanadate (green) using Phospho-Stat6 (Tyr641) antibody Stat6Y641-G12 at 0.1 μg/mL. Cat #orb1946356.

Flow cytometric analysis of U937 cells secondary antibody only negative control (blue) or untreated (red) or treated with IFNa and IL-4 (green) using 0.1 μg/mL Phospho-Stat6 (Tyr641) antibody Stat6Y641-G12. Cat. #orb1946356.

Western blot analysis of Daudi cell extract untreated or treated with IL-4 using 0.01 μg/mL Phospho-Stat6 (Tyr641) antibody Stat6Y641-G12 Cat. #orb1946356 or Company C antibody at 0.05 μg/mL (manufacturer's recommended concentration) developed using the same exposure.

Flow cytometric analysis of Ramos cells secondary antibody only negative control (blue) or untreated (red) or treated with IL-4 (green) using Phospho-Stat6 (Y641) antibody Stat6Y641-G12 (Cat. #orb1946356) or Company C antibody at 0.5 μg/mL (manufacturer's recommended concentration).

Quick Database Links

UniProt

P42226

UniProt Details

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No UniProt data available

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Protocol Information

Western Blot (IB, immunoblot)

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Flow Cytometry

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