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Unconjugated
SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE.
Predicted: 25.2 KDa. Observed: 32-45 KDa, reducing conditions
Mouse
Ile26-Arg233
C-6His
10 μg, 50 μg, 500 μg, 1 mgUnconjugated
SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE.
Predicted: 65.4 KDa. Observed: 85-100 KDa, reducing conditions
Human
Leu20-Pro365
C-Fc
50 μg, 500 μg, 1 mg, 10 μgBiotin
SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE.
Predicted: 20.9 KDa. Observed: 23-28 KDa, reducing conditions
Human
Glu108-Leu261
N-6His-Avi
20 μg, 100 μgUnconjugated
SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE.
Predicted: 39.2&38.1 KDa. Observed: 40-60 KDa, reducing conditions
Human
Asp23-Asp126&Gln23-Ser116
C-Fc-6His&C-Fc-Flag
10 μg, 50 μg, 500 μg, 1 mgUnconjugated
SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE.
Predicted: 103.7&81&22.7 KDa. Observed: 100-130&54 KDa, reducing conditions
Human
Glu31-Asn932
C-6His
10 μg, 50 μg, 500 μg, 1 mgUnconjugated
SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE.
Predicted: 72.2 KDa. Observed: 84-98 KDa, reducing conditions
Human
Gly24-Glu439
C-Fc
10 μg, 50 μg, 500 μg, 1 mgUnconjugated
SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE.
Predicted: 13.4 KDa. Observed: 25-40 KDa, reducing conditions
Human
Leu33-Arg143
C-6His
50 μg, 500 μg, 1 mg, 10 μgUnconjugated
SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE.
Predicted: 23.7 KDa. Observed: 19-30 KDa, reducing conditions
Human
Ala24-Asn239
C-6His
10 μg, 50 μg, 500 μg, 1 mgUnconjugated
SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE.
Predicted: 83.5 KDa. Observed: 90-110 KDa, reducing conditions
Human
Glu31-Thr542
C-Fc
10 μg, 50 μg, 500 μg, 1 mgUnconjugated
SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE.
Predicted: 36.1 KDa. Observed: 37 KDa, reducing conditions
Human
Phe113-Lys201
N-Fc
10 μg, 50 μg, 500 μg, 1 mg
