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Unconjugated
SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE.
Predicted: 52.3 KDa. Observed: 60-85 KDa, reducing conditions
Mouse
Val23-Gly258
C-Fc
10 μg, 50 μg, 500 μg, 1 mgUnconjugated
SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE.
Predicted: 28.7 KDa. Observed: 38-45 KDa, reducing conditions
Rhesus macaque
His27-Arg272
C-6His
10 μg, 50 μg, 500 μg, 1 mgUnconjugated
SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE.
Predicted: 54.8 KDa. Observed: 60-80 KDa, reducing conditions
Human
His27-Thr274
C-Fc
10 μg, 50 μg, 500 μg, 1 mgBiotin
SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE.
Predicted: 30.5 KDa. Observed: 40-60 KDa, reducing conditions
Human
His27-Thr274
C-Avi-6His
20 μg, 100 μgUnconjugated
SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE.
Predicted: 61.9 KDa. Observed: 80-110 KDa, reducing conditions
Mouse
Asp29-Leu348
C-mIgG2a Fc
500 μg, 1 mg, 10 μg, 50 μgBiotin
SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE.
Predicted: 27.9 KDa. Observed: 35-45 KDa, reducing conditions
Human
Gly42-Arg268
C-6His-Avi
20 μg, 100 μgBiotin
SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE.
Predicted: 24.9 KDa. Observed: 40-50 KDa, reducing conditions
Human
Lys22-Arg220
C-6His-Avi
20 μg, 100 μgBiotin
SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE.
Predicted: 19.3 KDa. Observed: 30-35 KDa, reducing conditions
Human
Ala26Pro180
C-6His-Avi
20 μg, 100 μgBiotin
SDS-PAGE: Greater than 90% as determined by reducing SDS-PAGE.
Predicted: 52.6 KDa. Observed: 58-62 KDa, reducing conditions
Human
Gln30-Gly254
C-Fc-Avi
20 μg, 100 μgBiotin
SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE.
Predicted: 50 KDa. Observed: 70-90 KDa, reducing conditions
Human
Gly24-His458
C-6His-Avi
20 μg, 100 μg
