Prohibitin Antibody / PHB

SKU: orb533836

Description

This antibody recognizes a protein of 30kDa which is identified as Prohibitin (PHB), an evolutionarily conserved protein with homologues found in yeast to man. It is located in the inner membrane of mitochondria. Although PHB mRNA and protein expression occurs throughout the cell cycle, maximum levels are detected during the G1/S phase transition and minimum levels are seen in S phase and the G2/mitosis boundary. Prohibitin is located exclusively in the mitochondria with the highest concentration on the inner membrane. It is an ideal mitochondrial marker. It shows anti-proliferative activity and has been proposed to play a role in normal cell cycle regulation, replicative senescence, cellular immortalization, and tumor suppression.

Images & Validation

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Tested Applications	IHC-P, WB
Dilution range	Western blot: 0.5-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT
Reactivity	Human
Application Notes	Optimal dilution of the Prohibitin antibody should be determined by the researcher.1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Key Properties

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Antibody Type	Primary Antibody
Host	Mouse
Clonality	Monoclonal
Isotype	Mouse IgG2c, kappa
Clone No.	PHB/1881
Immunogen	A recombinant human partial protein corresponding to amino acids 167-261 was used as the immunogen for the Prohibitin antibody.
Purification	Protein G affinity chromatography
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Disclaimer	For research use only

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Western blot testing of human 1) HEK293, 2) Jurkat, 3) HepG2 and 4) U-87 MG cell lysate with Prohibitin antibody (clone PHB/1881). Expected molecular weight ~30 kDa.

IHC testing of FFPE human liver tissue with Prohibitin antibody (clone PHB/1881). Required HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min followed by cooling at RT for 20 min.

SDS-PAGE Analysis of Purified, BSA-Free Prohibitin Antibody (clone PHB/1881). Confirmation of Integrity and Purity of the Antibody.

Western blot testing of human cell lysates with Prohibitin antibody (clone PHB/1881). Expected molecular weight ~30 kDa.

Western blot testing of human liver lysate with Prohibitin antibody (clone PHB/1881). Expected molecular weight ~30 kDa.

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UniProt

P35232

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No UniProt data available

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Protocol Information

Western Blot (IB, immunoblot)

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IHC-P

Immunohistochemistry Paraffin

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