Rabbit anti-EGFR Recombinant Monoclonal Antibody

SKU: orb1710837

Description

EGFR Antibody

Images & Validation

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Tested Applications	ICC, IHC, IP, WB
Dilution range	WB: 1:1,000, IP: 20 µl/1 mg lysate, IHC: 1:100 to 1:500. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections., ICC: 1:100 to 1:500. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE cell sections.
Reactivity	Human
Application Notes	Format: Whole IgG

Key Properties

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Antibody Type	Primary Antibody
Host	Rabbit
Clonality	Recombinant
Clone No.	BLR251L
Immunogen	Between 1100 and 1150
Target	EGFR
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/Appearance	Whole IgG
Buffer/Preservatives	Borate Buffered Saline (BBS) pH 8.2 with 0.1% rAlbumin and 0.09% Sodium Azide
Concentration	200 µg/ml
Disclaimer	For research use only

Alternative Names

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erb-b2 receptor tyrosine kinase 1; proto-oncogene c-ErbB-1; PIG61; NISBD2; receptor tyrosine-protein kinase erbB-1; HER1; ERBB; epidermal growth factor receptor tyrosine kinase domain; epidermal growth factor receptor; cell proliferation-inducing protein 61; cell growth inhibiting protein 40; avian erythroblastic leukemia viral (v-erb-b) oncogene homolog; mENA; ERBB1

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Rabbit anti-EGFR Recombinant Monoclonal Antibody

Detection of human EGFR by western blot. Samples: Whole cell lysate (50 µg) from GaMG, HeLa, OVCAR-8, MCF-7, and A-549 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-EGFR recombinant monoclonal antibody (orb1710837) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG. Detection: Chemiluminescence with an exposure time of 1 second. Lower Panel: Rabbit anti-Actin recombinant monoclonal antibody.

Detection of human EGFR by immunohistochemistry. Sample: FFPE section of human placenta. Antibody: Rabbit anti-EGFR recombinant monoclonal antibody (orb1710837). Secondary: HRP-conjugated goat anti-rabbit IgG.

Detection of human EGFR by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from OVCAR-8 cells prepared using NETN lysis buffer. Antibodies: Rabbit anti-EGFR recombinant monoclonal antibody (orb1710837) used for IP at 20 µl/mg lysate.

Detection of human EGFR by immunocytochemistry. Sample: FFPE section of A-431 cells. Antibody: Rabbit anti-EGFR recombinant monoclonal antibody (orb1710837). Secondary: HRP-conjugated goat anti-rabbit IgG.

Quick Database Links

Gene Symbol

EGFR

UniProt

P00533

UniProt Details

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No UniProt data available

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Protocol Information

Western Blot (IB, immunoblot)

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IHC

Immunohistochemistry

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ICC

Immunocytochemistry

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Immunoprecipitation

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