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Rabbit HIF1-alpha Recombinant Monoclonal Antibody
SKU: orb1520002
Description
Research Area
Cancer Biology, Cardiovascular Research, Immunology & Inflammation, Neuroscience
Images & Validation
−
| Tested Applications | ChIP, ICC, IHC, IP, WB |
|---|---|
| Dilution Range | WB - 1:1000; IP - 20 µl/mg lysate; IHC - 1:100 - 1:1,000. Epitope retrieval with citrate buffer pH 6.0 for 20 minutes using a pressure cooker is recommended for FFPE tissue sections.; ICC - 1:100 - 1:1,000. Epitope retrieval with citrate buffer pH 6.0 for 20 minutes using a pressure cooker is recommended for FFPE cell sections. |
| Reactivity | Human |
| Application Notes |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Recombinant |
| Isotype | IgG |
| Clone No. | BL-124-3F7 |
| Immunogen | Between 433 and 826 |
| Target | HIF1-alpha |
| Purification | Purified |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | 2 - 8°C |
|---|---|
| Form/Appearance | Liquid |
| Buffer/Preservatives | Borate Buffered Saline (BBS) pH 8.2 with 0.1% rAlbumin and 0.09% Sodium Azide |
| Concentration | 400 µg/ml |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−ARNT-interacting protein; basic-helix-loop-helix-PAS protein MOP1; bHLHe78; class E basic helix-loop-helix protein 78; HIF1; HIF-1A; HIF-1alpha; HIF-1-alpha; HIF1-ALPHA; hypoxia inducible factor 1 alpha subunit; hypoxia inducible factor 1, alpha subunit (basic helix-loop-helix transcription factor); hypoxia-inducible factor 1 alpha isoform I.3; hypoxia-inducible factor 1-alpha; hypoxia-inducible factor1alpha; member of PAS protein 1; member of PAS superfamily 1; MOP1; PAS domain-containing protein 8; PASD8
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Quick Database Links
UniProt Details
− No UniProt data available
NCBI Reference Sequences
−Associated Accession Numbers
Curated reference sequences for the gene transcript and protein product| Protein | NP_851397.1 |
|---|
Documents Download
Datasheet
Product Information
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC
Immunohistochemistry
ICC
Immunocytochemistry
IP
Immunoprecipitation
ChIP
Chromatin Immunoprecipitation
Rabbit HIF1-alpha Recombinant Monoclonal Antibody (orb1520002)
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