Rabbit MED1 Antibody

SKU: orb1529627

Description

MED1 Antibody

Research Area

Cancer Biology, Cardiovascular Research, Immunology & Inflammation, Metabolism Research, Neuroscience

Images & Validation

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Item 1 of 4

Tested Applications	IHC, IP, WB
Dilution Range	WB - 1:2,000 - 1:10,000; IP - 2 - 10 µg/mg lysate; IHC - 1:1,000 to 1:5,000. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.
Reactivity	Human, Mouse
Application Notes	Format: Whole IgG

Key Properties

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Antibody Type	Primary Antibody
Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Immunogen	Between 1531 and C-terminus
Target	MED1
Purification	Antigen Affinity Purified
Conjugation	Unconjugated

Storage & Handling

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Storage	2 - 8°C
Form/Appearance	Liquid
Buffer/Preservatives	Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Concentration	1000 µg/ml
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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activator-recruited cofactor 205 kDa component; ARC205; CRSP1; CRSP200; DRIP205; DRIP230; Mediator complex subunit 1; mediator of RNA polymerase II transcription subunit 1; p53 regulatory protein RB18A; PBP; peroxisome proliferator-activated receptor-binding protein; PPAR-binding protein; PPARBP; PPARGBP; RB18A; Thyroid receptor-interacting protein 2; TRAP220; TR-interacting protein 2; TRIP2; TRIP-2; vitamin D receptor-interacting protein 230 kD

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Detection of human MED1 by western blot.Samples: Whole cell lysate from HeLa, HEK293T, Jurkat, MCF-7, and Hep-G2 cells. Antibody: Affinity purified rabbit anti-MED1 antibody at 0.1 µg/ml.

Detection of mouse MED1 by western blot.Samples: Whole cell lysate from NIH 3T3, CT26, CH27, TCMK-1, and BW5147.3 cells. Antibody: Affinity purified rabbit anti-MED1 antibody at 0.1 µg/ml.

Detection of human MED1 by immunohistochemistry. Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti-MED1 antibody used at 1:5000 (0.2µg/ml). Secondary:HRP-conjugated goat anti-rabbit IgG.

Detection of human MED1 by western blot of immunoprecipitates.Samples: Whole cell lysate from HEK293T cells. Antibodies: Affinity purified rabbit anti-MED1 antibody.

Quick Database Links

Gene Symbol

MED1

UniProt

Q15648

RefSeq (Protein):NP_004765.2

UniProt Details

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No UniProt data available

NCBI Reference Sequences

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Associated Accession Numbers

Curated reference sequences for the gene transcript and protein product

Protein	NP_004765.2

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Protocol Information

Western Blot (IB, immunoblot)

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IHC

Immunohistochemistry

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Immunoprecipitation

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