Rabbit RPN2 Antibody

SKU: orb1523316

Description

RPN2 Antibody

Research Area

Metabolism Research

Images & Validation

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Item 1 of 4

Tested Applications	IHC, IP, WB
Dilution Range	WB - 1:1,000 - 1:5,000; IP - 2 - 10 µg/mg lysate; IHC - 1:500 - 1:2,000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
Reactivity	Human
Predicted Reactivity	Bovine, Mouse, Porcine, Rat
Application Notes	Format: Whole IgG

Key Properties

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Antibody Type	Primary Antibody
Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Immunogen	Between 375 and 425
Target	RPN2
Purification	Antigen Affinity Purified
Conjugation	Unconjugated

Storage & Handling

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Storage	2 - 8°C
Form/Appearance	Liquid
Buffer/Preservatives	Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Concentration	1000 µg/ml
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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dolichyl-diphosphooligosaccharide--protein glycosyltransferase 63 kDa subunit; dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 2; oligosaccharyltransferase complex subunit (non-catalytic); RIBIIR; Ribophorin II; ribophorin-2; RPNII; RPN-II; SWP1

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Detection of human RPN2 by western blot. Samples: Whole cell lysate (50 µg) from HeLa, HEK293T, and Jurkat cells. Antibodies: Affinity purified rabbit anti-RPN2 antibody orb1523316 used for WB at 0.4 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

Detection of human RPN2 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-RPN2 antibody orb1523316 used for IP at 6 µg per reaction.

Detection of human RPN2 by immunohistochemistry.Sample: FFPE section of human mammary glands. Antibody: Affinity purified rabbit anti-RPN2 (Cat. No. orb1523316) used at a dilution of 1: 1, 000 (1µg/ml). Detection: DAB

Detection of human RPN2 by immunohistochemistry.Sample: FFPE section of human prostate carcinoma. Antibody: Affinity purified rabbit anti-RPN2 (Cat. No. orb1523316) used at a dilution of 1: 1, 000 (1µg/ml). Detection: DAB

Quick Database Links

Gene Symbol

RPN2

UniProt

P04844

RefSeq (Protein):NP_002942.2

UniProt Details

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No UniProt data available

NCBI Reference Sequences

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Associated Accession Numbers

Curated reference sequences for the gene transcript and protein product

Protein	NP_002942.2

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Protocol Information

Western Blot (IB, immunoblot)

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IHC

Immunohistochemistry

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Immunoprecipitation

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