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Rabbit anti-SPIN90 Antibody
SKU: orb1526254
Description
Images & Validation
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| Tested Applications | IHC, IP, WB |
|---|---|
| Dilution range | WB - 1:2,000 - 1:10,000; IP - 2 - 10 µg/mg lysate; IHC - 1:200 - 1:1,000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections. |
| Reactivity | Human |
| Application Notes |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Immunogen | Between 672 and C-terminus |
| Target | SPIN90 |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | 2 - 8°C |
|---|---|
| Form/Appearance | Whole IgG |
| Buffer/Preservatives | Tris-buffered Saline containing 0.1% rAlbumin and 0.09% Sodium Azide |
| Concentration | 200 µg/ml |
| Disclaimer | For research use only |
Alternative Names
−WISH; ORF1; SH3 adapter protein SPIN90; SH3 protein interacting with Nck, 90 kDa; SPIN90; VIP54; WASP-interacting SH3-domain protein; wiskott-Aldrich syndrome protein-interacting protein; dia-interacting protein 1; NCK-interacting protein with SH3 domain; WASLBP; 90 kDa SH3 protein interacting with Nck; 54 kDa vimentin-interacting protein; AF3P21; dia interacting protein; Diaphanous protein-interacting protein; diaphanous protein interacting protein; 54 kDa VacA-interacting protein; DIP; DIP1; DIP-1
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Detection of human SPIN90 by western blot. Samples: Whole cell lysate (50 µg) from HeLa and HEK293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-SPIN90 antibody orb1526254 used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
Detection of human SPIN90 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-SPIN90 antibody orb1526254 used for IP at 6 µg per reaction.
Detection of human SPIN90 by immunohistochemistry.Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti-SPIN90 (Cat. No. orb1526254) used at a dilution of 1: 500 (0.4µg/ml). Detection: DAB
Quick Database Links
UniProt Details
− No UniProt data available
NCBI Reference Sequences
−Associated Accession Numbers
Curated reference sequences for the gene transcript and protein product| Protein | NP_057537.1 |
|---|
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Datasheet
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC
Immunohistochemistry
IP
Immunoprecipitation
Rabbit anti-SPIN90 Antibody (orb1526254)
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