Rabbit SPIN90 Antibody

SKU: orb1526254

Description

SPIN90 Antibody

Research Area

Cancer Biology, Immunology & Inflammation

Images & Validation

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Item 1 of 3

Tested Applications	IHC, IP, WB
Dilution range	WB - 1:2,000 - 1:10,000; IP - 2 - 10 µg/mg lysate; IHC - 1:200 - 1:1,000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
Reactivity	Human
Application Notes	Format: Whole IgG

Key Properties

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Antibody Type	Primary Antibody
Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Immunogen	Between 672 and C-terminus
Target	SPIN90
Purification	Antigen Affinity Purified
Conjugation	Unconjugated

Storage & Handling

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Storage	2 - 8°C
Form/Appearance	Liquid
Buffer/Preservatives	Tris-buffered Saline containing 0.1% rAlbumin and 0.09% Sodium Azide
Concentration	200 µg/ml
Disclaimer	For research use only

Alternative Names

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54 kDa VacA-interacting protein; 54 kDa vimentin-interacting protein; 90 kDa SH3 protein interacting with Nck; AF3P21; dia interacting protein; dia-interacting protein 1; diaphanous protein interacting protein; Diaphanous protein-interacting protein; DIP; DIP1; DIP-1; NCK-interacting protein with SH3 domain; ORF1; SH3 adapter protein SPIN90; SH3 protein interacting with Nck, 90 kDa; SPIN90; VIP54; WASLBP; WASP-interacting SH3-domain protein; WISH; wiskott-Aldrich syndrome protein-interacting protein

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Detection of human SPIN90 by western blot. Samples: Whole cell lysate (50 µg) from HeLa and HEK293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-SPIN90 antibody orb1526254 used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

Detection of human SPIN90 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-SPIN90 antibody orb1526254 used for IP at 6 µg per reaction.

Detection of human SPIN90 by immunohistochemistry.Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti-SPIN90 (Cat. No. orb1526254) used at a dilution of 1: 500 (0.4µg/ml). Detection: DAB

Quick Database Links

Gene Symbol

SPIN90

UniProt

Q9NZQ3

RefSeq (Protein):NP_057537.1

UniProt Details

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No UniProt data available

NCBI Reference Sequences

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Associated Accession Numbers

Curated reference sequences for the gene transcript and protein product

Protein	NP_057537.1

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Protocol Information

Western Blot (IB, immunoblot)

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IHC

Immunohistochemistry

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Immunoprecipitation

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