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Human CD3E

SKU: orb2994985

Description

Recombinant Human T-Cell Surface Glycoprotein CD3 Epsilon Chain is produced by our Mammalian expression system and the target gene encoding Asp23-Asp126 is expressed with a 6His tag at the C-terminus.

Images & Validation

Key Properties

Expression SystemHuman Cells
Biological OriginHuman
Biological ActivityELISA: Immobilized Human CD3E-His (Cat#orb2994985) at 10 μg/ml (100 μl/well) can bind Human Anti-CD3 mAb(Cat#orb2993159). The ED50 of Recombinant Human Anti-CD3 mAb is 3-15 ng/ml.
TagC-6His
Molecular WeightPredicted: 12.79 KDa. Observed: 15-21 KDa, reducing conditions
Expression RegionAsp23-Asp126
PuritySDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE.
Endotoxins< 1 EU/µg as determined by LAL test.
ConjugationUnconjugated

Storage & Handling

StorageLyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.
Form/AppearanceLyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.
ReconstitutionAlways centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
DisclaimerFor research use only

Alternative Names

T-Cell Surface Glycoprotein CD3 Epsilon Chain; T-Cell Surface Antigen T3/Leu-4 Epsilon Chain; CD3e; CD3E; T3E

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Human CD3E (orb2994985)

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Available Sizes

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10 μg
$ 150.00
50 μg
$ 390.00
500 μg
$ 1,790.00
1 mg
$ 2,540.00
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