Recombinant Human CD3E Protein

SKU: orb2994304

Description

Recombinant Human T-cell Surface Glycoprotein CD3 Epsilon Chain is produced by our Mammalian expression system and the target gene encoding Asp23-Asp126 is expressed with a human IgG1 Fc tag at the C-terminus.

Research Area

Immunology & Inflammation

Images & Validation

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Key Properties

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Expression System	Human Cells
Biological Origin	Human
Biological Activity	ELISA: Immobilized Human Human CD3E-Fc(Cat#orb2994304) at 2μg/ml (100 μl/well)can bind Anti-Human/Monkey CD3E mAb(Cat#orb2993159).The ED50 of Anti-Human/Monkey CD3E mAb(Cat#orb2993159) is 0.51ug/ml.
Tag	C-Fc
Expression Region	Asp23-Asp126
Endotoxins	< 1 EU/µg as determined by LAL test.
Conjugation	Unconjugated
MW	Predicted: 38.7 KDa. Observed: 40-50 KDa, reducing conditions
Purity	SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE.

Storage & Handling

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Storage	Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.
Form/Appearance	Lyophilized
Buffer/Preservatives	0.2 μm filtered solution of PBS, pH 7.4.
Reconstitution	Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
Expiration Date	6 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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T-Cell Surface Glycoprotein CD3 Epsilon Chain; T-Cell Surface Antigen T3/Leu-4 Epsilon Chain; CD3e; CD3E; T3E

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Quick Database Links

UniProt

P07766

UniProt Details

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No UniProt data available

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Protocol Information

Protein Handling and Storage Guide

Protein Handling Guide

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