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Recombinant Human Ciliary Neurotrophic Factor
Description
Images & Validation
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Key Properties
−| Expression System | Escherichia coli |
|---|---|
| Biological Activity | Testing in progress. |
| Molecular Weight | Approximately 22.9 kDa, a single non-glycosylated polypeptide chain containing 200 amino acids. |
| Protein Sequence | MAFTEHSPLT PHRRDLCSRS IWLARKIRSD LTALTESYVK HQGLNKNINL DSADGMPVAS TDQWSELTEA ERLQENLQAY RTFHVLLARL LEDQQVHFTP TEGDFHQAIH TLLLQVAAFA YQIEELMILL EYKIPRNEAD GMPINVGDGG LFEKKLWGLK VLQELSQWTV RSIHDLRFIS SHQTGIPARG SHYIANNKKM |
| Purity | > 97 % by SDS-PAGE and HPLC analyses. |
| Endotoxins | Less than 0.1 EU/ug of rHuCNTF as determined by LAL method. |
Storage & Handling
−| Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.- 12 months from date of receipt, -20 to -70 °C as supplied.- 1 month, 2 to 8 °C under sterile conditions after reconstitution.- 3 months, -20 to -70 °C under sterile conditions after reconstitution. |
|---|---|
| Form/Appearance | Lyophilized from a 0.2 μm filtered solution in PBS, pH 7.0, with 5 % Trehalose, 0.02 % Tween-20. |
| Disclaimer | For research use only |
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−Recombinant Human Ciliary Neurotrophic Factor [orb1906246]
> 97 % by SDS-PAGE and HPLC analyses.
Approximately 22.9 kDa, a single non-glycosylated polypeptide chain containing 200 amino acids.
Escherichia coli
100 μg, 500 μg, 5 μgHuman CNTF [orb3002368]
SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE. (QC verified). SEC-HPLC: Greater than 90% as determined by SEC-HPLC. (QC verified)
Predicted: 22.93 KDa. Observed: 25 KDa, reducing conditions
10 μg, 50 μg, 500 μg, 1 mgRecombinant Human Ciliary neurotrophic factor (CNTF) [orb2658983]
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Recombinant Human Ciliary Neurotrophic Factor (orb1952591)
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