Recombinant Human IL-27

SKU: orb2296569

Description

Images & Validation

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Tested Applications	ELISA, WB
Application Notes	Centrifuge the vial prior to opening, reconstitute in sterile distilled water or buffer of interest to concentration of 0.1-1mg/ml by gently pipetting 2-3 times, don't vortex

Key Properties

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Source	HEK293 cells
Reactivity	Human
Tag	C-terminal His Tag
Molecular Weight	60-65 kDa
Expression Region	Q14213（21-229） & Q8NEV9（29-243）
Purity	Greater than 90% as determined by SDS-PAGE

Storage & Handling

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Storage	The lyophilized protein is stable at -20°C for up to 1 year, the protein solution can be stored at 2-8°C for up to 1 week under sterile condition. For extended storage, it is recommended to further dilute in a buffer containing 0.1% BSA (make sure BSA will NOT interfere with your experiment ) and store in working aliquots at -20°C to -80°C for 6 months. Avoid repeated freeze/thaw cycle.
Buffer/Preservatives	Lyophilized from a 0.2 μm filtered solution in 10 mM Hepes, 500 mM NaCl with 5% trehalose, pH 7.4
Disclaimer	For research use only

Alternative Names

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IL 27, IL 27 A, IL 27 subunit alpha, IL 27A, IL27, IL-27, IL27 A, IL27A, IL27p28, IL30, interleukin 27, Interleukin 27 subunit

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Quick Database Links

UniProt

Q8NEV9, Q14213

UniProt Details

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No UniProt data available

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Protocol Information

Western Blot (IB, immunoblot)

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ELISA

Enzyme-linked Immunosorbent Assay (EIA)

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