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Recombinant Human Nuclear Antigen Antibody

SKU: orb2635156

Description

This monoclonal antibody is an excellent marker for human cells in xenographic model research. It reacts specifically with human cells. It is a part of a new panel of reagents, which recognizes subcellular organelles or compartments of human cells. These markers may be useful in identification of these organelles in cells, tissues, and biochemical preparations. MAb 235-1R recognizes an antigen associated with the nuclei in human cells. It can be used to stain the nuclei in cell or tissue preparations and can be used as a nuclear marker in subcellular fractions. It produces a speckled pattern in normal and malignant cells and may be used to stain the nuclei of cells in fixed or frozen tissue sections. It can also be used with paraformaldehyde fixed frozen tissue or cell preparations.

Images & Validation

Tested ApplicationsFACS, IF
Dilution RangeFlow cytometry: 1-2ug/million cells,Immunofluorescence: 1-3ug/ml
ReactivityHuman
Application Notes
Optimal dilution of the recombinant Human Nuclear Antigen antibody should be determined by the researcher.

Key Properties

Antibody TypePrimary Antibody
HostRabbit
ClonalityRecombinant
IsotypeRabbit IgG, kappa
Clone No.235-1R
ImmunogenNuclei of human myeloid leukemia biopsy cells were used as the immunogen for the recombinant Human Nuclear Antigen antibody.
PurificationProtein A/G affinity
ConjugationUnconjugated

Storage & Handling

StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/Preservatives1 mg/ml in 1X PBS; rAlbumin free, sodium azide free
Expiration Date12 months from date of receipt.
DisclaimerFor research use only

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Recombinant Human Nuclear Antigen Antibody

Immunofluorescent staining of MeOH-fixed human HeLa cells with recombinant Human Nuclear Antigen antibody (green, clone 235-1R).

Recombinant Human Nuclear Antigen Antibody

Immunofluorescent staining of PFA-fixed human HeLa cells recombinant Human Nuclear Antigen antibody (green, clone 235-1R).

Recombinant Human Nuclear Antigen Antibody

SDS-PAGE analysis of purified, BSA-free recombinant Human Nuclear Antigen antibody (clone 235-1R) as confirmation of integrity and purity.

UniProt Details

No UniProt data available

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Protocol Information

FACS
Fluorescence-Activated Cell Sorting (FC, Flow cytometry)
View Protocol
IF
Immunofluorescence
View Protocol

Recombinant Human Nuclear Antigen Antibody (orb2635156)

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Available Sizes

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100 μg
$ 750.00
DispatchUsually dispatched within 5-10 working days
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