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Recombinant Human Siglec-15 Protein
Description
Images & Validation
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Key Properties
−| Expression System | Human Cells |
|---|---|
| Biological Origin | Human |
| Biological Activity | BLI: Loaded Human Siglec-15-Fc(at#orb2994021) on AHC Biosensor, can bind Anti-Human Siglec15 mAb-mFc(Cat#orb2993156) with an affinity constant of 0.13 nM as determined in BLI assay. (Regularly tested) |
| Tag | C-Fc |
| Molecular Weight | Predicted: 53.5 KDa. Observed: 50-80 KDa, reducing conditions |
| Expression Region | Phe20-Thr263 |
| Purity | SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE. |
| Endotoxins | < 1 EU/µg as determined by LAL test. |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
|---|---|
| Form/Appearance | Lyophilized |
| Buffer/Preservatives | 0.2 μm filtered solution of PBS, 150mM NaCl, 0.3% Chaps, 5% Trehalose, pH 7.4. |
| Reconstitution | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
| Expiration Date | 6 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−Similar Products
−Recombinant Human Siglec-15 Protein (Biotin) [orb2993902]
Biotin
SDS-PAGE: Greater than 90% as determined by reducing SDS-PAGE.. SEC-HPLC: Greater than 90% as determined by SEC-HPLC.(QC verified)
Predicted: 55.3 KDa. Observed: 58-75 KDa, reducing conditions
20 μg, 100 μgRecombinant Cynomolgus Siglec-15 Protein [orb2993817]
Unconjugated
SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE.
Predicted: 53.1 KDa. Observed: 55-70 KDa, reducing conditions
1 mg, 10 μg, 50 μg, 500 μgRecombinant Human Siglec-15 Protein [orb2994025]
Unconjugated
SDS-PAGE: Greater than 90% as determined by reducing SDS-PAGE.
Predicted: 52.1 KDa. Observed: 50-70 KDa, reducing conditions
10 μg, 50 μg, 500 μg, 1 mgRecombinant Human Siglec-15 Protein [orb2994040]
Unconjugated
SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE.
Predicted: 27.4 KDa. Observed: 30-40 KDa, reducing conditions
10 μg, 50 μg, 500 μg, 1 mg

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Recombinant Human Siglec-15 Protein (orb2994021)
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