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Recombinant human TNF alpha Antibody

SKU: orb2657852

Description

Recombinant human TNF alpha Antibody

Research Area

Immunology & Inflammation

Images & Validation

Tested ApplicationsFunctional Studies
Application Notes
Recombinant protein TNF alpha is expressed in S2 cell (Drosophila). DNA sequence encoding 157 AAs was fused with Strep-tag at the N-terminus. A calculated molecular mass of recombinant protein is 18.7 kDa.

Key Properties

Antibody TypeRecombinant Antibody
ClonalityRecombinant
PurificationPurified by affinity chromatography. Lyophilized from a 0.2 μm filtered solution in Storage buffer (100 mM Tris, 150 mM NaCl, 0.01% Tween 20, pH 8.0).
ConjugationUnconjugated

Storage & Handling

StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Expiration Date12 months from date of receipt.
DisclaimerFor research use only

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Recombinant human TNF alpha Antibody

Recombinant Human TNF alpha induces cytotoxicity in HeLa cell line in the presence of the protein synthesis inhibitor cycloheximide. Measured in WST-8 fluorometric assay method. The ED50 for this effect is 4.2513 ng/mL.

Recombinant human TNF alpha Antibody

Recombinant Human TNF alpha induces cytotoxicity in HeLa cell line in the presence of the protein synthesis inhibitor cycloheximide. Measured in WST-8 fluorometric assay method. The ED50 for this effect is 4.2513 ng/mL.

Recombinant human TNF alpha Antibody

Recombinant Human TNF alpha induces cytotoxicity in HeLa cell line in the presence of the protein synthesis inhibitor cycloheximide. Measured in WST-8 fluorometric assay method. The ED50 for this effect is 4.2513 ng/mL.

NCBI Gene Details

No NCBI Gene data available

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Recombinant human TNF alpha Antibody (orb2657852)

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0.05 mg
$ 300.00
Free Secondary Antibody (20 ul)0/0

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