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Recombinant Human TROP-2 Protein
Description
Images & Validation
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Key Properties
−| Expression System | Human Cells |
|---|---|
| Biological Origin | Human |
| Biological Activity | ELISA: Immobilized Human TROP-2-Fc(Cat#orb2994108) at 2μg/ml (100 μl/well) can bind Anti-Human TROP-2 mAb* (Cat#orb2993115).*: Biotinylated by NHS-biotin prior to testing.The ED50 of Anti-Human TROP-2 mAb* (Cat#orb2993115) is 5.16 ng/ml. |
| Tag | C-Fc |
| Molecular Weight | Predicted: 48.7 KDa. Observed: 60-80 KDa, reducing conditions |
| Expression Region | Thr88-Thr274 |
| Purity | SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE. |
| Endotoxins | < 1 EU/µg as determined by LAL test. |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
|---|---|
| Form/Appearance | Lyophilized |
| Buffer/Preservatives | 0.2 μm filtered solution of PBS, pH 7.4. |
| Reconstitution | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
| Expiration Date | 6 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
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−Recombinant Human TROP-2 Protein [orb2993744]
Unconjugated
SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE.
Predicted: 54.8 KDa. Observed: 60-80 KDa, reducing conditions
10 μg, 50 μg, 500 μg, 1 mgRecombinant Human TROP-2 Protein (Biotin) [orb2993745]
Biotin
SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE.
Predicted: 30.5 KDa. Observed: 40-60 KDa, reducing conditions
20 μg, 100 μgRecombinant Human TROP-2 Protein [orb2993769]
Unconjugated
SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE.. SEC-HPLC: Greater than 95% as determined by SEC-HPLC. (QC verified)
Predicted: 28.9 KDa. Observed: 40-50 KDa, reducing conditions
10 μg, 50 μg, 500 μg, 1 mgRecombinant Human TROP-2 Protein [orb2994102]
Unconjugated
SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE.
Predicted: 22.6 KDa. Observed: 28-40 KDa, reducing conditions
10 μg, 50 μg, 500 μg, 1 mgRecombinant Human Tumor-associated calcium signal transducer 2 (TACSTD2), partial (Active) [orb1674362]
Greater than 95% as determined by SDS-PAGE.
30.6 kDa
Mammalian cell
1 mg, 100 μg

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Recombinant Human TROP-2 Protein (orb2994108)
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