Recombinant SARS-CoV-2 Nucleoprotein

SKU: orb1952703

Description

Images & Validation

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Tested Applications	ELISA, WB
Application Notes	Centrifuge the vial prior to opening, reconstitute in sterile distilled water or buffer of interest to concentration of 0.1-1mg/ml by gently pipetting 2-3 times, don't vortex

Key Properties

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Source	E.Coli
Reactivity	Virus
Tag	N-terminal His-EGFP Tag
Molecular Weight	76.7 kDa
Expression Region	1-419
Purity	Greater than 95% as determined by SDS-PAGE

Storage & Handling

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Storage	The lyophilized protein is stable at -20°C for up to 1 year, the protein solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing 0.1% BSA (make sure BSA will NOT interfere with your experiment ) and store in working aliquots at -20°C to -80°C for 6 months. Avoid repeated freeze/thaw cycle.
Buffer/Preservatives	Lyophilized from a 0.2 μm filtered solution in 10 mM Hepes, 500 mM NaCl with 5% trehalose, pH 7.4
Disclaimer	For research use only

Alternative Names

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2019-nCoV nucleocapsid protein, NC, Nucleoprotein, Protein N

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Quick Database Links

UniProt

P0DTC9

UniProt Details

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No UniProt data available

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Protocol Information

Western Blot (IB, immunoblot)

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ELISA

Enzyme-linked Immunosorbent Assay (EIA)

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