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Description
Research Area
Images & Validation
−| Tested Applications | ELISA |
|---|---|
| Dilution Range | Used for specific, high-sensitivity detection of SARS-CoV-2 nucleocapsid protein (NP) in immunoassay. Can be paired with other NP-specific clones for detection in sandwich ELISA format. |
| Reactivity | Other |
Key Properties
−| Host | Rabbit |
|---|---|
| Clonality | Monoclonal |
| Isotype | Rabbit IgG1k |
| Clone No. | B06E6C |
| Immunogen | SARS-CoV-2 nucleoprotein (NP) specific peptide |
| Purification | Protein A |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Form/Appearance | Liquid |
| Buffer/Preservatives | 1X PBS, 0.02% NaN3, 50% Glycerol, 0.1% rAlbumin |
| Concentration | 0.5 mg/mL |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
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Western blot analysis of SARS-CoV2 and SARS-CoV nucleocapsid protein (50 ng) probed with 1 ug/mL CoV2NP rabbit monoclonal antibody (84C4a), CoV2NP-84C4. Cat. #orb1945990.

Microtiter wells were coated with SARS-CoV-2 (COVID-19) Nucleocapsid Protein (NP) and SARS-CoV NP at 1 ug/mL. Purified rabbit monoclonal antibody 84C4a (Cat# orb1945990) was serially diluted 1:2 starting at 1 ug/mL, and shows very strong and specific binding to COVID-19 NP antigen, with no significant cross-reactivity to SARS-CoV NP antigen.

A sandwich ELISA was performed using SARS-CoV-2 (COVID-19) Nucleocapsid Protein (NP) specific rabbit monoclonal antibodies: 84C4a (Cat# orb1945990) as a capture antibody and 75G5a-biotin (Cat# orb1945991) as a detection antibody. COVID-19 NP was serially diluted 1:2 starting at 25 ng/mL. Rabbit monoclonal antibodies 84C4a and 75G5a-biotin detected COVID-19 NP antigen at very high sensitivity as low as 49 pg/mL (4.9 pg).

Affinity measurement of SARS-CoV-2 (84C4a) antibody.
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SARS-CoV-2 Nucleoprotein (NP) rabbit mAb Antibody (orb1945990)
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