SARS-CoV-2 NP (75G5a) rabbit mAb Biotin Conjugate Antibody

SKU: orb1945991

Description

SARS-CoV-2 NP (75Ga) rabbit mAb Biotin Conjugate for immunoassay. Biorbyt creates robustly validated, high quality recombinant mAbs.

Images & Validation

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Tested Applications	ELISA
Dilution range	Used for specific, high-sensitivity detection of SARS-CoV-2 nucleocapsid protein (NP) in immunoassay. Can be paired with other NP-specific clones for detection in sandwich ELISA format.
Reactivity	Other

Key Properties

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Host	Rabbit
Clonality	Monoclonal
Isotype	Rabbit IgG1k
Clone No.	75G5a
Immunogen	SARS-CoV-2 nucleoprotein (NP) specific peptide
Purification	Protein A
Conjugation	Biotin

Storage & Handling

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Storage	2-8°C
Form/Appearance	Liquid
Buffer/Preservatives	1X PBS, 0.02% NaN3
Concentration	0.5 mg/mL
Disclaimer	For research use only

Alternative Names

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Severe acute respiratory syndrome coronavirus 2 nucleoprotein, 2019-nCoV nucleocapsid protein

Quality Guarantee

Explore bioreagents carefree to elevate your research. All our products are rigorously tested for performance. If a product does not perform as described on its datasheet, our scientific support team will provide expert troubleshooting, a prompt replacement, or a refund. For full details, please see our Terms & Conditions and Buying Guide. Contact us at support@biorbyt.com.

SARS-CoV-2 NP (75G5a) rabbit mAb Biotin Conjugate Antibody

Microtiter wells were coated with SARS-CoV-2 (COVID-19) Nucleocapsid Protein (NP) at 1 ug/mL. Biotin-conjugated rabbit monoclonal antibody 75G5a (Cat# orb1945991) was serially diluted 1:2 starting at 1 ug/mL, and shows high-sensitivity binding to COVID-19 NP antigen.

Western blot analysis of SARS-CoV-2 and SARS-CoV nucleocapsid protein (50 ng) probed with 1 ug/mL CoV2NP nucleocapsid rabbit monoclonal antibody (75G5), CoV2NP-75G5a), Cat# orb1945992.

A sandwich ELISA was performed using SARS-CoV-2 (COVID-19) Nucleocapsid Protein (NP) specific rabbit monoclonal antibodies: 84C4a (Cat# orb1945990) as a capture antibody and 75G5a-biotin (Cat# orb1945991) as a detection antibody. COVID-19 NP was serially diluted 1:2 starting at 25 ng/mL. Rabbit monoclonal antibodies 84C4a and 75G5a-biotin detected COVID-19 NP antigen at very high sensitivity as low as 49 pg/mL (4.9 pg).

A sandwich ELISA was performed using SARS-CoV-2 (COVID-19) Nucleocapsid Protein (NP) specific rabbit monoclonal antibodies: 85C1 (Cat# orb1945986) as a capture antibody and 75G5a-biotin (Cat# orb1945991) as a detection antibody. COVID-19 NP was serially diluted 1:2 starting at 25 ng/mL. Rabbit monoclonal antibodies 85C1 and 75G5a-biotin detected COVID-19 NP antigen at very high sensitivity as low as 25 pg/mL (2.5 pg).

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UniProt

P0DTC9

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Protocol Information

ELISA

Enzyme-linked Immunosorbent Assay (EIA)

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