RecombinantEGF,Human

SKU: orb1494894

Description

Epidermal Growth Factor (EGF) is a potent growth factor that stimulates the proliferation of various epidermal and epithelial cells. Additionally, EGF has been shown to inhibit gastric secretion, and to be involved in wound healing. EGF signals through a receptor known as c-erbB, which is a class I tyrosine kinase receptor. This receptor also binds with TGF-α and VGF (vaccinia virus growth factor). Recombinant human EGF is a 6.2 kDa globular protein containing 53 amino acid residues including 3 intramolecular disulfide-bonds. Recombinant human Epidermal Growth Factor (rhEGF) produced in E. coli is a non-glycosylated polypeptide chain of 54 amino acids. A fully biologically active molecule, rhEGF has a molecular mass of 6.2kDa analyzed by reducing SDS-PAGE and is obtained by proprietary refolding and chromatographic techniques at GenScript.

Images & Validation

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Application Notes	Reconstituted in ddH2O at 100 μg/ml.

Key Properties

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Source	Escherichia coli.
Biological Activity	ED50 1.7 x 10ˆ6 units/mg.
Molecular Weight	6.2kDa, observed by reducing SDS-PAGE.
Protein Sequence	MNSDSECPL SHDGYCLHDG VCMYIEALDK YACNCVVGYI GERCQYRDLK WWELR
Purification	> 95% by SDS-PAGE analysis.
Purity	> 95% by SDS-PAGE analysis.
Endotoxins	Less than 0.1 ng/µg (1 EU/μg) determined by LAL test

Storage & Handling

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Storage	Lyophilized recombinant human Epidermal Growth Factor (rhEGF) remains stable up to 12 months at -80°C from date of receipt. Upon reconstitution, rhEGF should be stable up to 4 weeks at 4°C or up to 6 months at -20°C.
Form/Appearance	Lyophilized from a 0.2 μm filtered solution in 10mM PB, pH 7.0
Buffer/Preservatives	Lyophilized from a 0.2 μm filtered solution in 10mM PB, pH 7.0
Disclaimer	For research use only

Alternative Names

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Epidermal Growth Factor

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