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RecombinantEGF,Human
SKU: orb1494895
Description
Images & Validation
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| Application Notes |
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Key Properties
−| Source | Escherichia coli. |
|---|---|
| Biological Activity | The ED50, calculated by the dose-dependant proliferation of murine BALB/c 3T3 cells is less then 2 ng/ml, corresponding to a specific activity of 5.0×10ˆ5 IU/ mg. |
| Molecular Weight | 6.0 kDa+/-10% determined by reduced SDS-PAGE |
| Protein Sequence | MNSDSECPLS HDGYCLHDGV CMYIEALDKY ACNCVVGYIG ERCQYRDLKW WELR |
| Purification | Greater than 95% as determined by (a) Analysis by SEC-HPLC(b) Analysis by reducing and non-reducing SDS-PAGE Silver Stained gel |
| Purity | Greater than 95% as determined by (a) Analysis by SEC-HPLC(b) Analysis by reducing and non-reducing SDS-PAGE Silver Stained gel |
| Endotoxins | Less than 0.1ng/μg (1 EU/μg) of recombinant human Epidermal Growth Factor (EGF) as determined by LAL test. |
Storage & Handling
−| Storage | Lyophilized recombinant Human Epidermal Growth Factor (rhEGF) remains stable up to 12 months at -80°C from date of receipt. Upon reconstitution, rhEGF should be stable up to 4 weeks at 4°C or up to 6 months at -20°C. |
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| Form/Appearance | Recombinant human Epidermal Growth Factor (EGF) was lyophilized after extensive dialysis against 10mM Phosphate buffer, pH 7.0, 200mM NaCl buffer. |
| Buffer/Preservatives | Recombinant human Epidermal Growth Factor (EGF) was lyophilized after extensive dialysis against 10mM Phosphate buffer, pH 7.0, 200mM NaCl buffer. |
| Disclaimer | For research use only |
Alternative Names
−Epidermal Growth Factor
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−RecombinantEGF,Human [orb1494894]
> 95% by SDS-PAGE analysis.
6.2kDa, observed by reducing SDS-PAGE.
Escherichia coli.
100 μg, 500 μg, 1 mg
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RecombinantEGF,Human (orb1494895)
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