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Browse validated feedback from researchers employing Biorbyt products.
Phospho-CREB1 signals were robustly evident, revealing striking differences in phosphorylation levels between groups. The assay demonstrated stable reproducibility and is well-suited for investigating learning, memory, and synaptic plasticity.
Western blot detection of phospho-CREB1 (Ser142) using a specific antibody. A single band at the predicted molecular weight (~43 kDa) confirms specificity, with low background and a high signal-to-noise ratio.
The antibody specifically recognized a target band at 43 kDa with high clarity. The observed migration pattern accurately matched the theoretical molecular weight of the phosphorylated epitope.
Western blot analysis using the anti-c-Myc antibody detected a distinct, specific band at approximately 60 kDa, consistent with the predicted molecular weight. The band exhibited high clarity without noticeable tailing or smearing, and nonspecific background signals were minimal.
The anti-Myc antibody performed exceptionally well, detecting a band at the predicted molecular weight with high specificity and excellent reproducibility.
Probing lysates with the Biorbyt Bax antibody sample revealed a single, specific band at ~21 kDa, consistent with the predicted molecular weight and free of significant smearing. The blot displayed a clean background and negligible nonspecific reactivity. Upon chemiluminescent detection, the bands were sharp and well-defined, with reliable experimental reproducibility, confirming the antibody's suitability for detecting apoptosis-related proteins.
Western blot analysis using the c-Myc antibody detected a clear primary band at 57 kDa, consistent with the predicted molecular weight. The assay exhibited low background, minimal nonspecific bands, and robust reproducibility across repeated experiments.
Western blot analysis using the biorbyt Bax antibody detected a single, distinct band at approximately 21 kDa, consistent with the predicted molecular weight. The assay exhibited minimal nonspecific binding, a clean background, a high signal-to-noise ratio, and excellent band contrast.
A clear single band was detected at approximately 21 kDa by Western blot (WB) using the Bax antibody, with accurate target localization. Few non-specific bands were observed, resulting in a clean background. The assay showed a high signal-to-noise ratio, strong contrast after development, and good reproducibility, making it suitable for the detection and study of apoptosis-related proteins.
Western blot analysis showing a clear, single band at the predicted size (1:1000 dilution), with low background and rapid chemiluminescence detection.
Working well! I used this antibody for immunohistochemistry using human and organotypic 3D skin, and found this Ab was working. This antibody recognized AHR in both cytoplasm and nucleus. I used 1:150 dilution as a primary Ab.
Performance of the antibody is satisfactory without much variability.Effectiveness- This antibody detected primary mouse osteoclast grown in culture. Specificity- This antibody specifically detected osteoclast progenitor population and expression down regulated in mature osteoclast population. Quality and reproducibility- The quality and reproducibility of the antibody is quite satisfactory. Dilution- For immunofluorescence staining in cells the dilution used as 1:200. Issues- Punctate staining…
Satisfactory service from Biorbyt. Used on untreated and heat treated FFPE sections of human tonsil and mouse lymph node. Antibody concentrations of orb156399 tested were 10, 2 and 0.4µg/mL ON @ 4°C. Used with ABC-HRP and double-IF with XCL1 which resulted in some dbl+ but gave also many other unexpected single-CLEC9a positive cells. Our collaborator contacted Biorbyt and received another antibody.
I tested the antibody on human ovarian cancer cell extracts and A549 cell line. Samples were collected, sonicated, mixed with loading buffer, and resolved on a vertical electrophoresis system using denaturating conditions. I incubated the nitrocellulose membrane using 1:1000 antibody diluted in 5% non-fat milk with TBS-T for 2 h on rt, washed in TBS-T and incubated with adequate secondary antibody. Visualization was done using ECL. Signals were strong, but there was three of them, between the…
Performs well! I used P-mtor in my western blot protocole The probing of p mtor is a little bad and didnt give clear bands after revelations After probing the membrane many times,its give a clear bands but with a little background.
