RNase A (DNase free)

SKU: orb532708

Description

RNase A is an endoribonuclease that attacks at the 3'-phosphate of a pyrimidine nucleotide. The sequence of pG-pG-pC-pA-pG will be cleaved to give pG-pG-pCp and A-pG. The highest activity is exhibited with ssRNA. RNase A is free of detectable DNase and protease activity, a heat treatment of the enzyme is not necessary before use. Stability: RNase A is an extremely stable enzyme, remarkable resistant to heating. It renatures easily after treatment with most denaturing agents.

Images & Validation

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Application Notes	Applications: 1) Plasmid and genomic DNA preparation 2) Removal of RNA from recombinant protein preparations 3) Ribonuclease protection assays 4) Mapping single-base mutations in DNA or RNA.

Key Properties

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Biological Activity	≥ 80 Kunitz units/mg
CAS Number	9001-99-4
MW	Theoretical MW: 13.7 kDa (monomer)
Purity	≥ 90% (ion exchange chromatography), salt free, chromatographically homogeneous lyophilisate

Customer Validated Data

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The data below is submitted by researchers worldwide. We provide this transparency to help you decide if this product works for your specific application or species, even if we haven't tested it ourselves.

Success by Application

Reactivity Distribution

Latest Experiments

2 Results

Dilution

Sample

Summary

In vitro

Human

100 μg/mL

Fixed melanoma cell suspensions (SKMEL28, MALME3M, A375, G361, UACC62, MELST, HEK293FT)

RNase A was used in cell...

In vitro

Human

0.8 mg/mL

Cultured human cells (H1299 cell line)

RNase A was used as a...

Storage & Handling

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Storage	store at 2-8 °C
Form/Appearance	dry powder
Concentration	Working concentration: 1-100 µg/ml (depending on application). The enzyme is active under a wide range of reaction conditions. At low salt concentrations (0 to 100 mM NaCl), RNase cleaves ss and dsRNA as well as the RNA strand in RNA-DNA hybrids.
Expiration Date	6 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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Endoribonuclease, Bovine pancreas

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Protocol Information

Protein Handling and Storage Guide

Protein Handling Guide

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