(S)-10-Hydroxycamptothecin

SKU: orb1305050

Description

(S)-10-Hydroxycamptothecin (10-HCPT) is a potent topoisomerase I inhibitor used in cancer research. It demonstrates significant antitumor activity in both in vitro cell assays and in vivo models, particularly in studies focused on hepatoma and other solid tumors.

Research Area

Cell Biology, Molecular Biology

Images & Validation

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Key Properties

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CAS Number	19685-09-7
MW	364.35
Purity	99.81% (May vary between batches)
Formula	C₂₀H₁₆N₂O₅
SMILES	O=C1N2C(C=3C(C2)=CC=4C(N3)=CC=C(O)C4)=CC5=C1COC(=O)[C@@]5(CC)O
Target	Apoptosis,Topoisomerase
Solubility	DMSO:26 mg/mL (71.36 mM);H2O:< 1 mg/mL (insoluble or slightly soluble);Ethanol:< 1 mg/mL (insoluble or slightly soluble)

Bioactivity

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In Vivo	(S)-10-Hydroxycamptothecin inhibits cell growth in BT-20 cells (IC50=34.3 nM) and MDA-231 cells (IC50=7.27 nM), demonstrating its efficacy. It also induces the formation of cleavable complexes mediated by human topoisomerase I in pBR322 plasmid DNA (EC50=0.35 μM). Additionally, in human microvascular endothelial cells (HMEC), (S)-10-Hydroxycamptothecin dose-dependently inhibits cell growth (IC50=0.31 μM), significantly hampers HMEC migration (IC50=0.63 μM), and impedes angiogenesis (IC50=0.96 μM). Read more...
In Vitro	In the CAM model, 10-Hydroxycamptothecin (25 nM) inhibits angiogenesis in a concentration-dependent manner. In mice carrying Colo 205 xenografts, oral administration of 10-Hydroxycamptothecin (2.5-7.5 mg/kg) every two days significantly suppresses tumor cell growth. Read more...
Cell Research	Cells are exposed to various concentrations of 10-Hydroxycamptothecin for 72 hours. Cell growth is monitored by the standard MTT assay.(Only for Reference) Read more...

Storage & Handling

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Storage	Powder: -20°C for 3 years \| In solvent: -80°C for 1 year \| Shipping with blue ice/Shipping at ambient temperature.
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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PCNA, p53, G2/M, Cell-cycle, c-Myc, (S) 10 Hydroxycamptothecin, 10-HCPT, 10-Hydroxycamptothecin, (S)10Hydroxycamptothecin, caspase-1/3r, Bcl-2, Apoptosis, antitumor, arrest, Bax, Topoisomerase, Topo I

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Compound Identifiers

PubChem CID

97226 ↗

Key Properties

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No computed properties available.

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