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SARS-CoV-2 (COVID-19) ORF7a Antibody
SKU: orb1239059
Featured
Description
Research Area
Infectious Disease & Virology
Images & Validation
−Item 1 of 2
| Tested Applications | ELISA, IHC |
|---|---|
| Reactivity | Virus |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | Anti-SARS-CoV-2 (COVID-19) ORF7a antibody (orb1239059) was raised against a peptide corresponding to 15 amino acids near the central domain of SARS-CoV-2 (COVID-19) ORF7a protein. The immunogen is located within 40-90 amino acids of the SARS-CoV-2 (COVID-19) ORF7a protein. |
| Target | ORF7a protein, Accessory protein 7a, Protein U122, Protein X4, ORF7a |
| Molecular Weight | 14kD |
| Purification | SARS-CoV-2 (COVID-19) ORF7a Antibody is affinity chromatography purified via peptide column. |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Form/Appearance | Liquid |
| Buffer/Preservatives | SARS-CoV-2 (COVID-19) ORF7a antibody is supplied in PBS containing 0.02% sodium azide. |
| Concentration | 1 mg/mL |
| Disclaimer | For research use only |
Alternative Names
−ORF7a protein, Accessory protein 7a, Protein U122, Protein X4, ORF7a
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SARS-CoV-2 (COVID-19) ORF7a protein polyclonal antibody [orb1272801]
ELISA
Virus
Rabbit
Polyclonal
Unconjugated
0.1 mg
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Immunohistochemistry Validation of SARS-CoV-2 (COVID-19) ORF7a in COVID-19 Patient Lung. Immunohistochemical analysis of paraffin-embedded COVID-19 patient lung tissue using anti-SARS-CoV-2 (COVID-19) ORF7a antibody (orb1239059, 1 µg/mL). Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4 ˚C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin. Strong signal of SARS-COV-2 ORF7a protein was observed in macrophages of COVID-19 patient lung, but not in non-COVID-19 patient lung.
ELISA Validation. Antibodies: SARS-CoV-2 (COVID-19) ORF7a Antibody, orb1239059. A direct ELISA was performed using SARS-CoV-2 ORF7a immunogen peptide (orb1231669) as coating antigen and the anti-SARS-CoV-2 (COVID-19) ORF7a antibody as the capture antibody. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:20000 dilution. Detection range is from 0.3 ng/mL to 1000 ng/mL.
Quick Database Links
UniProt Details
− No UniProt data available
NCBI Reference Sequences
−Associated Accession Numbers
Curated reference sequences for the gene transcript and protein product| RefSeq | P0DTC7.1 |
|---|
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Protocol Information
IHC
Immunohistochemistry
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
SARS-CoV-2 (COVID-19) ORF7a Antibody (orb1239059)
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