SARS-CoV-2 (COVID-19) Spike S2 Antibody

SKU: orb1238694

Description

Images & Validation

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Item 1 of 4

Tested Applications	ELISA, IF, IHC, WB
Reactivity	Virus

Key Properties

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Antibody Type	Primary Antibody
Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Immunogen	Anti-SARS-CoV-2 (COVID-19) Spike S2 antibody (orb1238694) was raised against a peptide corresponding to 16 amino acids near the carboxy terminus of SARS-CoV-2 (COVID-19) Spike glycoprotein. The immunogen is located within 1130-1180 amino acids of SARS-CoV-2 (COVID-19) Spike protein.
Target	S
Purification	SARS-CoV-2 (COVID-19) Spike Antibody is affinity chromatography purified via peptide column.
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/Appearance	Liquid
Buffer/Preservatives	SARS-CoV-2 (COVID-19) Spike Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration	1 mg/mL
Disclaimer	For research use only

Alternative Names

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SARS-CoV-2 (COVID-19) Spike Antibody: Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), Surface Glycoprotein, Spike protein

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Immunohistochemistry Validation of SARS-CoV-2 (COVID-19) Spike S2 in COVID-19 Patient Lung. Immunohistochemical analysis of paraffin-embedded COVID-19 patient lung tissue using anti-SARS-CoV-2 (COVID-19) Spike S2 antibody (orb1238694, 0.1 µg/mL). Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4 ˚C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin. Strong signal of SARS-COV-2 spike protein was observed in macrophage of COVID-19 patient lung, but not in non-COVID-19 patient lung.

ELISA Validation with SARS-CoV-2 (COVID-19) Spike S2 ECD Recombinant Protein. Antibodies: SARS-CoV-2 (COVID-19) Spike S2 antibody, orb1238694. A direct ELISA was performed using SARS-CoV-2 (COVID-19) Spike S2 ECD recombinant protein (orb1231609) as coating antigen and the anti-SARS-CoV-2 (COVID-19) Spike S2 antibody as the capture antibody. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:20000 dilution. Detection range is from 8 ng/mL to 1000 ng/mL.

Western Blot Validation with SARS-CoV-2 (COVID-19) Spike S2 ECD Recombinant Protein. Loading: 30 ng per lane of SARS-CoV-2 (COVID-19) Spike S2 ECD recombinant protein, orb1231609. Antibodies: SARS-CoV-2 (COVID-19) Spike S2, orb1238694, 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution. Lane 1: 1 µg/mL and Lane 2: 2 µg/mL.

Overexpression Validation in Spike Transfected 293 Cells. Loading: 10 µg per lane of Spike transfected 293 cell lysate. Antibodies: SARS-CoV-2 (COVID-19) Spike, orb1238694, 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution. Lane 1: 1 µg/mL and Lane 2: 2 µg/mL.

Quick Database Links

Gene Symbol

UniProt

P0DTC2

RefSeq:QHD43416

UniProt Details

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No UniProt data available

NCBI Reference Sequences

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Associated Accession Numbers

Curated reference sequences for the gene transcript and protein product

RefSeq	QHD43416

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Protocol Information

Western Blot (IB, immunoblot)

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IHC

Immunohistochemistry

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Immunofluorescence

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ELISA

Enzyme-linked Immunosorbent Assay (EIA)

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