SARS-CoV-2 Nucleocapsid Antibody

SKU: orb1274616

Description

Images & Validation

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Item 1 of 3

Tested Applications	ELISA, IHC, WB
Reactivity	Virus
Application Notes	ELISA, IHC, WB; FC (Other applications as reported in literature)

Key Properties

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Antibody Type	Primary Antibody
Clonality	Recombinant
Isotype	Mouse IgG2a
Clone No.	1C7C7
Immunogen	Recombinant nucleoprotein of SARS-CoV (severe acute respiratory syndrome).The nucleocapsid protein is expressed in the internal nucleocapsid of SARS-CoV-2.
Purification	>95% by SDS Page
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/Appearance	Liquid
Buffer/Preservatives	This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (PBS) pH 7.2 - 7.4, 150 mM NaCl with no carrier protein, potassium, calcium or preservatives added.
Concentration	>1.0 mg/ml
Disclaimer	For research use only

Alternative Names

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SARS-CoV-2 Nucleocapsid, SARS-CoV-2 Nucleoprotein, Protein N, SARS-CoV N Protein

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Vero E6 infectedcwith SARS-CoV-2, Wa-1 @ MOI = 0.1 for 48 hours. Cells were fixed with 4 % formaldehyde (4hr), perm 0.1% Triton X (15 min), blocked with 4% BSA, 1:1000 diluted with clone 1C7C7 (Anti-SARS-CoV-2 Nucleocaspid) O/N tehn 1:1000 anti-mouse Dylight 488 (1 hour).

Lysates were derived from A549 cells (Transduced with ACE2 by adenoviral vector), mock vs. SARS-CoV-2-infected MO12, 24 hours, with 60ug of lysates loaded/lane.

Exp1293 cells were transfected with expression plasmid coding for SARs2 NP and incubated for 48 hours. Cells were permeabilized, incubated with dilutions of supermatant collected from cells transfected with DNA plasmids coding for the heavy and light chains of 1C7 and binding identified by the addition of goat anti-mouse IgG FITC.

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Protocol Information

Western Blot (IB, immunoblot)

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IHC

Immunohistochemistry

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ELISA

Enzyme-linked Immunosorbent Assay (EIA)

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