SARS-CoV-2 Spike P681H Antibody (Alpha, Mu Variant)

SKU: orb1238673

Description

Images & Validation

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Item 1 of 3

Tested Applications	ELISA, WB
Reactivity	Virus

Key Properties

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Antibody Type	Primary Antibody
Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Immunogen	Anti-SARS-CoV-2 (COVID-19) P681H Mutant Specific Spike P681H Antibody (orb1238673) was raised against a peptide corresponding to 12 amino acids near the center of SARS-CoV-2 UK variant (B.1.1.7) Spike protein. The immunogen is located within 650-700 amino acids of SARS-CoV-2 UK variant (B.1.1.7) Spike protein.
Target	S
Purification	SARS-CoV-2 (COVID-19) Alpha Variant (B.1.1.7, UK) Spike P681H Antibody is Protein A purified.
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/Appearance	Liquid
Buffer/Preservatives	SARS-CoV-2 (COVID-19) P681H Mutant Specific Spike antibody is supplied in PBS containing 0.02% sodium azide.
Concentration	1 mg/mL
Disclaimer	For research use only

Alternative Names

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SARS-CoV-2 (COVID-19) Spike Antibody: Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), Surface Glycoprotein, Spike protein

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SARS-CoV-2 Spike P681H Antibody (Alpha, Mu Variant)

ELISA Validation of P681H Mutant Specific Spike Antibodies with SARS-CoV-2 UK Variant Spike S1 Protein. Coating Antigen: SARS-CoV-2 spike S1 proteins, including WT and UK variant (B.1.1.7), 1 µg/mL, incubate at 4 ˚C overnight. Detection Antibodies: SARS-CoV-2 UK Variant Spike antibody, orb1238673, dilution: 0.1-1000 ng/mL, incubate at RT for 1 hr. Secondary Antibodies: Goat anti-rabbit HRP at 1:20000, incubate at RT for 1 hr. SARS-CoV-2 P681H Mutant Specific Spike antibody (orb1238673) can specifically detect UK variant spike S1 protein, but not WT spike S1 protein (orb1231589).

WB Validation of P681H Mutant Specific Spike Antibodies with SARS-CoV-2 UK Variant Spike S1 Protein. Loading: 50 ng of SARS-CoV-2 spike S1 proteins, including WT and UK variant (B.1.1.7). Detection Antibodies: SARS-CoV-2 P681H Mutant Specific Spike antibody, orb1238673, 1 µg/mL, incubate at RT for 1 hr. Secondary Antibodies: Goat anti-rabbit HRP at 1:20000, incubate at RT for 1 hr. SARS-CoV-2 P681H Mutant Specific Spike antibody (orb1238673) can specifically detect UK variant spike S1 protein, but not WT spike S1 protein (orb1231589).

ELISA Validation of P681H Mutant Specific Spike Antibodies with Mutant and WT Peptide. Coating Antigen: SARS-CoV-2 spike S1 peptides, including WT and UK variant (B.1.1.7), 1 µg/mL, incubate at 4 ˚C overnight. Detection Antibodies: SARS-CoV-2 P681H Mutant Specific Spike antibody, orb1239981, dilution: 0.1-1000 ng/mL, incubate at RT for 1 hr. Secondary Antibodies: Goat anti-rabbit HRP at 1:20000, incubate at RT for 1 hr. Immunogen region of antibody orb1239981 includes site 681P that was mutated in B.1.1.7. SARS-CoV-2 P681H Mutant Specific Spike antibody (orb1238673) can specifically detect UK variant spike S1 peptide (681H), but not WT peptide (681P).

Quick Database Links

Gene Symbol

UniProt

P0DTC2

RefSeq:QHD43416

UniProt Details

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No UniProt data available

NCBI Reference Sequences

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Associated Accession Numbers

Curated reference sequences for the gene transcript and protein product

RefSeq	QHD43416

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Protocol Information

Western Blot (IB, immunoblot)

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ELISA

Enzyme-linked Immunosorbent Assay (EIA)

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