Sheep IgG (H&L) Antibody Alkaline Phosphatase Conjugated

SKU: orb347930

Description

Sheep IgG (H&L) antibody (Alkaline Phosphatase)

Images & Validation

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Item 1 of 1

Tested Applications	DOT, ELISA, IHC, WB
Dilution range	ELISA: 1:5,000, IHC: 1:200 - 1:1,000, WB: 1:500 - 1:2,000
Reactivity	Sheep
Application Notes	Anti-Sheep IgG (H&L) Alkaline Phosphatase has been tested by ELISA and dot blot and is suitable for use in immunoelectrophoresis, western-blot, competitive western-blot, ELISA and competitive ELISA assays. Specific conditions for reactivity and signal detection should be optimized by the end user.

Key Properties

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Antibody Type	Secondary Antibody
Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Immunogen	Anti-Sheep IgG (H&L) was produced by repeated immunization with Sheep IgG whole molecule in rabbit.
Purity	This product was prepared from monospecific antiserum by immunoaffinity chromatography using Sheep IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Alkaline Phosphatase (calf intestine), anti-Rabbit Serum, Sheep IgG and Sheep Serum.
Conjugation	AP

Storage & Handling

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Storage	Store vial at 4° C before opening. DO NOT FREEZE. This product is stable at 4° C as an undiluted liquid. Dilute only prior to immediate use. Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity.
Form/Appearance	Liquid (sterile filtered)
Buffer/Preservatives	0.1% (w/v) Sodium Azide
Concentration	1.0 mg/mL
Disclaimer	For research use only

Alternative Names

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Rabbit Anti-Sheep IgG alkaline phosphatase Conjugated Antibody, Rabbit Anti-Sheep IgG Antibody alk phos Conjugation

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Sheep IgG (H&L) Antibody Alkaline Phosphatase Conjugated

Dot Blot of Rabbit anti-Sheep IgG antibody Alkaline Phosphatase Conjugated. Antigen: Sheep IgG. Load: 200 ng, 66.7 ng, 22.2 ng, 7.41 ng or 2.47 ng as indicated. Primary antibody: N/A. Secondary antibody: Alkaline Phosphatase Conjugated Rabbit anti-Sheep IgG Antibody. Block: Blocking Buffer for Fluorescent Western Blotting (orb348637) at RT for 1 hour.

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Protocol Information

Western Blot (IB, immunoblot)

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IHC

Immunohistochemistry

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ELISA

Enzyme-linked Immunosorbent Assay (EIA)

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DOT

Dot Blot

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