Shh Rabbit Polyclonal Antibody

SKU: orb11368

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Description

Images & Validation

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Item 1 of 4

Tested Applications	IF, IHC-Fr, IHC-P, WB
Dilution range	WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
Reactivity	Mouse, Rat
Predicted Reactivity	Bovine, Equine, Gallus, Human, Rabbit

Related Conjugates & Formulations

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Key Properties

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Antibody Type	Primary Antibody
Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Immunogen	KLH conjugated synthetic peptide derived from human Shh (21-120/462aa)
Target	SHH
Molecular Weight	19/47 kDa
Purification	Affinity purified by Protein A
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/Appearance	Liquid
Buffer/Preservatives	0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
Concentration	1mg/ml
Disclaimer	For research use only

Alternative Names

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Sonic hedgehog; Vhh-1; Sonic hedgehog protein; SHH; HHG 1; HHG1; HLP 3; HLP3; Holoprosencephaly 3; HPE 3; HPE3; MCOPCB5; SMMC I; SMMCI; Sonic Hedgehog(Drosophila) homolog; sonic hedgehog homolog(Drosophila); Sonic hedgehog homolog; Sonic hedgehog protein; TPT; TPTPS; Sonic hedgehog protein N-product; SHH_HUMAN.

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Sizes Available: 100 μl

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Paraformaldehyde-fixed, paraffin embedded (Mouse stomach), Antigen retrieval by microwave in sodium citrate buffer (pH6.0), Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes, Blocking buffer (3% BSA) at RT for 30 min, Antibody incubation with (Shh) Polyclonal Antibody, Unconjugated (orb11368) at 1:400 overnight at 4°C, followed by conjugation to the secondary antibody (labeled with HRP) and DAB staining.

Paraformaldehyde-fixed, paraffin embedded (rat stomach), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (Shh) Polyclonal Antibody, Unconjugated (orb11368) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Sample: Embryo (Mouse) tissue at 30 ug, Primary: Anti-Shh (orb11368) at 1:300 dilution, Secondary: HRP conjugated Goat Anti-Rabbit IgG (orb572747) at 1:5000 dilution, Predicted band size: 19/47kD, Observed band size: 50kD.

Sample: Stomach (Mouse) Lysate at 40 ug, Primary: Anti-Shh (orb11368) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 19/47kD, Observed band size: 49 kD.