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Thioredoxin rabbit pAb

SKU: orb771709
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Description

Rabbit polyclonal antibody to TRX

Images & Validation

Tested ApplicationsELISA, IHC-P, WB
Dilution rangeIHC-p: 100-300.WB 1:500-2000, ELISA 1:10000-20000
ReactivityHuman, Mouse, Rat

Key Properties

ClonalityPolyclonal
IsotypeIgG
ImmunogenSynthesized peptide derived from human Thioredoxin. at AA range: 52-101
Molecular Weight12
PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
ConjugationUnconjugated

Storage & Handling

StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/PreservativesPBS with 0.02% sodium azide and 50% glycerol pH 7.4.
Concentration1 mg/ml
DisclaimerFor research use only

Alternative Names

Anti-Thioredoxin (Trx) (ATL-derived factor) (ADF) (Surface-associated sulphydryl protein) (SASP) antibody

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Thioredoxin rabbit pAb

Western Blot analysis of 1, mouse-liver 2, hela cells using primary antibody diluted at 1:1000 (4°C overnight). Secondary antibody:Goat Anti-rabbit IgG IRDye 800 ( diluted at 1:5000, 25°C, 1 hour) .

Thioredoxin rabbit pAb

Immunohistochemical analysis of paraffin-embedded Human skin. 1, Antibody was diluted at 1:400 (4°C overnight). 2, High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 30min).

Thioredoxin rabbit pAb

Immunohistochemical analysis of paraffin-embedded Human skin. 1, Antibody was diluted at 1:400 (4°C overnight). 2, High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 30min).

Thioredoxin rabbit pAb

Immunohistochemical analysis of paraffin-embedded Human skin. 1, Antibody was diluted at 1:400 (4°C overnight). 2, High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 30min).

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Protocol Information

WB
Western Blot (IB, immunoblot)
View Protocol
IHC-P
Immunohistochemistry Paraffin
View Protocol
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
View Protocol

Thioredoxin rabbit pAb (orb771709)

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Available Sizes

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50 μl
$ 250.00
100 μl
$ 380.00
DispatchUsually dispatched within 5-10 working days
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