TNF alpha Antibody

SKU: orb1333677

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Description

Mouse monoclonal antibody to TNF alpha (TNF)

Images & Validation

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Item 1 of 3

Tested Applications	IHC, WB
Dilution Range	WB 1:4000, IHC 1:150
Reactivity	Human
Application Notes	For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Key Properties

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Antibody Type	Primary Antibody
Host	Mouse
Clonality	Monoclonal
Isotype	IgG2a
Clone No.	OTI1D9
Immunogen	Full length human recombinant protein of human TNF(NP_000585) produced in HEK293T cell.
Target	TNF alpha
Molecular Weight	25.5 kDa
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

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HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY TNF (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-TNF.

Immunohistochemical staining of paraffin-embedded Human lymph node tissue within the normal limits using anti-TNF mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris, pH8.5, 120°C for 3min)

Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-TNF mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris, pH8.5, 120°C for 3min)

Quick Database Links

Gene Symbol

TNF alpha

UniProt

P01375

RefSeq (mRNA):NM_000594

UniProt Details

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No UniProt data available

NCBI Reference Sequences

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Associated Accession Numbers

Curated reference sequences for the gene transcript and protein product

mRNA	NM_000594

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Protocol Information

Western Blot (IB, immunoblot)

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IHC

Immunohistochemistry

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