TNF alpha antibody

SKU: orb371962

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Description

TNF alpha antibody

Images & Validation

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Item 1 of 7

Tested Applications	ICC, IF, IHC-P
Dilution range	IF/ICC: 1:100-800, WB: 1:100-2000, IHC-P: 1:100-800 (based on 0.5 mg/ml)
Reactivity	Human, Mouse, Rat

Key Properties

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Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Immunogen	KLH conjugated synthetic peptide derived from human TNF alpha. Please contact us for the exact immunogen sequence. The peptide is available as orb374984.
Target	TNF alpha
Molecular Weight	27 kDa
Purity	Polyclonal antibodies are purified by peptide affinity chromatography
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/Appearance	10 mM PBS, 0.02% sodium azide
Concentration	- 100 μg (in 200 μl): 0.5 mg/ml- 200 μg (in 400 μl): 0.5 mg/ml
Disclaimer	For research use only

Alternative Names

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anti-APC1 antibody, anti-Cachectin antibody, anti-DIF antibody, anti-Differentiation inducing factor antibody, anti-MCF antibody, anti-Necrosin antibody, anti-TNF a antibody, anti-TNF alpha antibody, anti-TNF antibody, anti-TNF Monocyte Derived antibody, anti-TNF-a antibody, anti-TNF-alpha antibody, anti-TNFA antibody, anti-Tumor necrosis factor alpha antibody, anti-TNF-alpha antibody

Quality Guarantee

Explore bioreagents carefree to elevate your research. All our products are rigorously tested for performance. If a product does not perform as described on its datasheet, our scientific support team will provide expert troubleshooting, a prompt replacement, or a refund. For full details, please see our Terms & Conditions and Buying Guide. Contact us at support@biorbyt.com.

IHC-P image iof human breast tumour using TNF alpha antibody (2.5 ug/ml)

Immunofluorescense analysis of rat skin tissue using TNF alpha antibody (2.5 ug/ml)

Immunohistochemical staining of rat skin tissue using TNF alpha antibody (2.5 ug/ml)

IF analysis of mouse uterus tissue using anti-TNF alpha (2.5 ug/ml)

Immunohistochemical staining of mouse uterus tissue using TNF alpha antibody (2.5 ug/ml)

Immunofluorescense analysis of rat skin tissue using TNF alpha antibody (2.5 ug/ml)

Immunohistochemical staining of paraffin embedded rat liver tissue using anti-TNF alpha (2.5 ug/ml)

Quick Database Links

Gene Symbol

TNF alpha

UniProt

P06804, P16599, P01375

NCBI Gene ID

7124

RefSeq:000585, 000594

UniProt Details

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No UniProt data available

NCBI Gene Details

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No NCBI Gene data available

NCBI Reference Sequences

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Associated Accession Numbers

Curated reference sequences for the gene transcript and protein product

RefSeq	000585, 000594

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Protocol Information

IHC-P

Immunohistochemistry Paraffin

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Immunofluorescence

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ICC

Immunocytochemistry

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IHC (2)

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Human (2)

H Keyhan, H Morovvati, MK Koohi, J Hassan, M Mazidi Hematological and Histological Changes in the Knee Cartilage of Male Rats Received Intra-Articular Injection of Bee Venom and Melittin journals.ut.ac.ir, (2025)

PubMed:

Mehmet Berker 1, Sibel Köktürk 1, Sibel Doğan 1, Emel Usta Myricetin ameliorates the effects of hydrogen peroxide-induced oxidative stress in human mesenchymal stem cells: an ultrastructural and immunocytochemical study Ultrastruct Pathol, (2025)

PubMed: 40247639

Applications

IHC

Reactivity

Human

Mahshid Kharaziha 1 2, Sahar Salehi 2, Mahshid Shokri 1, Seyed Mohsen Ahmadi Tafti 3 4, Thomas Scheibel 2 5 6 7 8 ROS-Scavenging Multifunctional Microneedle Patch Facilitating Wound Healing Adv Healthc Mater, (2025)

PubMed: 40832795

Applications

IHC

Reactivity

Human