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Description
Research Area
Immunology & Inflammation
Images & Validation
−Item 1 of 1
| Tested Applications | ELISA, WB |
|---|---|
| Application Notes |
Key Properties
−| Source | HEK293 cells |
|---|---|
| Biological Activity | Measured by its binding ability in a functional ELISA. Immobilized Human TNF-alpha, His Tag at 0.2 ug/mL (100 uL/well) can bind Human TNFR1, Fc Tag with a linear range of 0.4-6.25 ng/mL. |
| Target | TNF-alpha |
| Tag | His Tag |
| MW | 18.2 kDa |
| Purity | >95% as determined by SDS-PAGE.Endotoxin level is less than 1.0 EU per ug by the LAL method. |
| Protein Sequence | Val 77 - Leu 233 |
Storage & Handling
−| Storage | Lyophilized Protein should be stored at -20°C or lower for long term storage. Upon reconstitution, working aliquots should be stored at -20°C or -70°C. Avoid repeated freeze-thaw cycles. |
|---|---|
| Form/Appearance | Lyophilized |
| Buffer/Preservatives | PBS, pH 7.4 |
| Expiration Date | 6 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−DIF,TNF-alpha,TNFA,TNFSF2,cachexin,cachectin,TNFalpha
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Immobilized Human TNF-alpha, His Tag at 0.2 ug/ml (100 uL/well) can bind Human TNFR1, Fc Tag with a linear range of 0.4-6.25 ng/mL.
Quick Database Links
Gene Symbol
TNF-alpha
RefSeq (Protein):NP_000585.2
NCBI Reference Sequences
−Associated Accession Numbers
Curated reference sequences for the gene transcript and protein product| Protein | NP_000585.2 |
|---|
Documents Download
Datasheet
Product Information
Request a Document
Protocol Information
WB
Western Blot (IB, immunoblot)
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
TNF-alpha Recombinant Protein (orb1227719)
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