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TNF alpha Recombinant Protein
SKU: orb1229855
Description
Research Area
Cancer Biology, Cell Biology, Immunology & Inflammation, Metabolism Research, Signal Transduction
Images & Validation
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| Application Notes |
|---|
Key Properties
−| Source | E. coli |
|---|---|
| Biological Activity | ED50 is less than 0.03 ng/ml as determined by the cytolysis of murine L929 cells in the presence of Actinomycin D.Specific Activity of 3.0 x 107 IU/mg. |
| Target | TNF |
| Tag | Tag Free |
| Molecular Weight | 17.3 kD |
| Protein Sequence | Val77-Leu233 |
| Purity | Greater than 95% as determined by reducing SDS-PAGE.Endotoxin level less than 0.1 ng/ug (1 IEU/ug) as determined by LAL test. |
Storage & Handling
−| Storage | Lyophilized protein should be stored at -20°C, though stable at room temperature for 3 weeks.Reconstituted protein solution can be stored at 4-7°C for 2-7 days.Aliquots of reconstituted samples are stable at -20°C for 3 months. |
|---|---|
| Form/Appearance | Lyophilized |
| Buffer/Preservatives | Lyophilized from a 0.2 um filtered solution of 20mM PB, 150mM NaCl, pH 7.0. It is not recommended to reconstitute to a concentration less than 100 ug/ml. Dissolve the lyophilized protein in ddH2O. |
| Expiration Date | 6 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−Tumor Necrosis Factor, Cachectin, TNF-Alpha, Tumor Necrosis Factor Ligand Superfamily Member 2, TNF-a, TNF, TNFA, TNFSF2
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TNF alpha Recombinant Protein (orb1229855)
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