You have no items in your shopping cart.
TNFSF11 Antibody
SKU: orb1240016
Featured
Description
Research Area
Cell Biology
Images & Validation
−Item 1 of 6
| Tested Applications | ELISA, IHC-P, WB |
|---|---|
| Reactivity | Human, Mouse, Rat |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | Anti-sRANK-L antibody (orb1240016) was raised against a peptide corresponding to 14 amino acids near the center of human sRANK-L. The immunogen is located within amino acids 140-190 of sRANK-L. |
| Target | TNFSF11 |
| Molecular Weight | Predicted: 36kDObserved: 36 kD |
| Purification | sRANK Ligand Antibody is affinity chromatography purified via peptide column. |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Form/Appearance | Liquid |
| Buffer/Preservatives | sRANK Ligand Antibody is supplied in PBS containing 0.02% sodium azide. |
| Concentration | 1 mg/mL |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−sRANK Ligand Antibody: ODF, OPGL, sOdf, CD254, OPTB2, RANKL, TRANCE, hRANKL2, Tumor necrosis factor ligand superfamily member 11, Osteoclast differentiation factor, ODF
Similar Products
−- Item 1 of 5
RANKL/CD254 Rabbit Polyclonal Antibody [orb11190]
FC, IF, IHC-Fr, IHC-P, WB
Canine, Rat
Human, Mouse
Rabbit
Polyclonal
Unconjugated
100 μl, 50 μl, 200 μl - Item 1 of 4
- Item 1 of 4
RANKL rabbit pAb Antibody [orb767009]
ELISA, IF, IHC, WB
Human, Mouse, Rat
Polyclonal
Unconjugated
100 μl - Item 1 of 3
RANK Rabbit Polyclonal Antibody [orb312842]
FC, ICC
Mouse, Rat
Human
Rabbit
Polyclonal
Unconjugated
50 μl, 100 μl, 200 μl - Item 1 of 6
Quality Guarantee
Explore bioreagents carefree to elevate your research. All our products are rigorously tested for performance. If a product does not perform as described on its datasheet, our scientific support team will provide expert troubleshooting, a prompt replacement, or a refund. For full details, please see our Terms & Conditions and Buying Guide. Contact us at support@biorbyt.com.
Western Blot Validation in Human Cell Lines. Loading: 15 µg of lysates per lane. Antibodies: sRANK-L orb1240016 (1 µg/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
Western Blot Validation in Mouse Tissues. Loading: 15 µg of lysates per lane. Antibodies: sRANK-L orb1240016 (2 µg/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
Western Blot Validation with Recombinant Protein. Loading: 30 ng of human sRANK-L recombinant protein per lane. Antibodies: sRANK-L orb1240016 (A: 1 µg/mL, B: 2 µg/mL and C: 4 µg/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution. Observed at around 27kD.
Western Blot Validation in Rat Liver Tissue. Loading: 15 µg of lysates per lane. Antibodies: sRANK-L orb1240016 (A: 0.25 µg/mL and B: 0.5 µg/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
Immunohistochemistry Validation of sRANK-L in Human Liver Tissue. Immunohistochemical analysis of paraffin-embedded human liver tissue using anti sRANK-L antibody (orb1240016) at 5 µg/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4°C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.
Immunohistochemistry and Induced Expression Validation of sRANK-L in Rat Osteogenic Compartment (Hassan et al., 2017). Protein analysis for sRANK-L by immunohistochemistry with anti-sRANK-L antibodies (orb1240016) in rat osteogenic cells. Strong immunoreactivity is shown for sRANK-L in osteogenic cells at 18hr after upper jaw molar extraction as compared to baseline.
Documents Download
Datasheet
Product Information
Request a Document
Protocol Information
WB
Western Blot (IB, immunoblot)
IHC-P
Immunohistochemistry Paraffin
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
TNFSF11 Antibody (orb1240016)
- 0.0
Based on 0 reviews
Participating in our Biorbyt product reviews program enables you to support fellow scientists by sharing your firsthand experience with our products.
Login to Submit a ReviewAvailable Sizes
Select a size below
Usually dispatched within 5-10 working days