Y-27632

SKU: orb1307291

Description

ZY-27632 is a potent, cell-permeable inhibitor of ROCK-I and ROCK-II kinases. It is widely used in vitro to study cytoskeletal dynamics and cell adhesion, and in vivo to investigate its role in apoptosis prevention, such as in isolated prostate progenitor cells.

Research Area

Cell Biology, Signal Transduction, Stem Cell & Developmental Biology

Images & Validation

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Key Properties

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CAS Number	146986-50-7
MW	247.34
Purity	99.87% (May vary between batches)
Formula	C₁₄H₂₁N₃O
SMILES	C[C@@H](N)[C@H]1CC[C@@H](CC1)C(=O)Nc1ccncc1
Target	ROCK,Apoptosis
Solubility	DMSO:127.5 mg/mL (515.48 mM);H2O:insoluble;10% DMSO+90% Saline:2.48 mg/mL (10.03 mM)

Bioactivity

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Target IC50	PKA:25 μM (Ki)\|PKCα:73 μM (Ki)\|PKN:3.1 μM (Ki)\|ROCK2:300 nM(Ki)\|ROCK1:220 nM(Ki)\|CITK:5.3 μM (Ki)\|Citron kinase:5.3 μM (Ki)
In Vivo	METHODS: To investigate the therapeutic potential of Y-27632 in motor neuron disease, Y-27632 (2 or 30 mg/kg in drinking water) was administered orally to SOD1G93A mice in the ALS model for 137 days. RESULTS: Y-27632 2 mg/kg treatment was ineffective, Y-27632 30 mg/kg treatment improved motor function in male mice, and female mice showed only limited improvement. METHODS: To investigate the effect of Y-27632 on liver fibrosis, Y-27632 (30 mg/kg) was administered orally to rats with dimethylnitrosamine (DMN)-induced liver fibrosis once a day for four weeks. RESULTS: Y-27632 treatment significantly reduced the incidence of DMN-induced hepatic fibrosis and lowered the levels of collagen and hydroxyproline as well as the expression of α-SMA in the liver. Read more...
In Vitro	METHODS: Human induced pluripotent stem cells, marmoset iPSC, were treated with Y-27632 (5-20 μM) for 7 days and clone formation was detected by AKP. RESULTS: Y-27632 significantly improved the cloning efficiency of marmoset iPSC. METHODS: Adult adipose tissue-derived stem cells ADSCs were treated with Y-27632 (5 μmol/L) for 1 h. The morphological changes of ADSCs were detected. RESULTS: Y-27632 dose-dependently induced neuronal differentiation in ADSCs. the percentage of neuron-like cells in ADSCs treated with 5 μmol/L Y-27632 for 1 h was (93.5±4.7)%. METHODS: Crab monkey embryonic stem cells cyES were routinely passaged or treated with Y-27632 (1-10 μM) for 24 h. Live-dead staining was performed using the Flow Cytometry method, and BrdU was detected using a kit. RESULTS: Y-27632 promoted the increase of cyES surviving cells. Y-27632 did not promote cell proliferation, but protected the cells from cell death after single-cell digestion. Read more...
Cell Research	Y-27632 is dissolved in water and stored. HeLa cells are plated at a density of 3×104 cells per 3.5-cm dish. The cells are cultured in DMEM containing 10% FBS in the presence of 10 mM Thymidine for 16 h. After the cells are washed with DMEM containing 10% FBS, they are cultured for an additional 8 h, and then 40 ng/mL of Nocodazole is added. After 11.5 h of the Nocodazole treatment, various concentrations of Y-27632 (0-300 μM), Y-30141, or vehicle is added and the cells are incubated for another 30 min. Read more...

Storage & Handling

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Storage	keep away from direct sunlight \| Powder: -20°C for 3 years \| In solvent: -80°C for 1 year \| Shipping with blue ice/Shipping at ambient temperature.
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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Apoptosis, inhibit, Inhibitor, Y27632, Y-27632, Y 27632, Rho-associated protein kinase, Rho-kinase, Rho-associated kinase, ROK, ROCK2, ROCK1, ROCK

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Compound Identifiers

PubChem CID

448042 ↗

Key Properties

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No computed properties available.

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Cell Culture (1)

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Human (1)

Jane In den Birken et al. Investigating the Anticancer Effects of Sulforaphane in an In Vitro Coculture Model of Prostate Cancer Cells with Engineered Heart Tissue ACS Pharmacol Transl Sci, (2025)

PubMed: 41409171

Nicola Wanner et al. Lysine-specific histone demethylase 1a regulates nephron development and long-term transcriptional programming JCI Insight, 11(5), e190283 (2026)

PubMed: 41797715

Applications

Cell Culture

Reactivity

Human

Y-27632 (orb1307291)

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Purity:	99.98% (May vary between batches)
CAS Number:	129830-38-2
MW:	320.26
Formula:	C₁₄H₂₁N₃O·2HCl

Purity:	>98%
CAS Number:	129830-38-2
MW:	320.26
Formula:	C₁₄H₂₁N₃O · 2HCl

Purity:	99.98%
CAS Number:	331752-47-7
MW:	338.27
Formula:	C₁₄H₂₅Cl₂N₃O₂

CAS Number:	199433-55-1
MW:	286.37
Formula:	C₁₆H₂₂N₄O

Purity:	>98%(HPLC)
CAS Number:	146986-50-7
MW:	247.3
Formula:	C₁₄H₂₁N₃O

Y-27632

Description

Research Area

Images & Validation

Key Properties

Bioactivity

Storage & Handling

Alternative Names

Similar Products

Y-27632 dihydrochloride [orb1307611]

Y-27632 2HCl [orb546031]

Y-27632 HCl hydrate [orb1740051]

Y-30141 [orb2814710]

Y27632 [orb1226217]

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Y-27632 (orb1307291)

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