Yadanzigan

SKU: orb3054382

Description

Yadanzigan (YDZG) is an anti-inflammatory agent and a NLRP3 inhibitor. Yadanzigan specifically inhibits NLRP3 activation via inhibiting NF-κB pathway and Reactive Oxygen Species production. Yadanzigan also moderates LPS -induced acute lung injury (ALI) in mice.

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Key Properties

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CAS Number	76588-87-9
MW	574.6
Purity	>98% (HPLC)
Formula	C₂₆H₃₈O₁₄

Bioactivity

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In Vivo

Animal model: Male BABL/C mice (18-22 g). Dosage: 5 mg/kg, 10 mg/kg. Administration: Intravenous injection; dose at 2 h prior to and 6 h, 12 h after LPS induction. Result: Reduced LPS-induced infiltration of inflammatory cells and cytokine levels, such as TNF-α and IL-6. Inhibited NLRP3 inflammasome activation in lung tissue. Resulted significantly greater survival rate.

In Vitro

Western blot analysis. Cell line: J774A.1, and THP-1 cells Concentration: For J774A.1: 2.5 μM, 5 μM, and 10 μM; For THP-1: 10 μM, 20 μM, and 40 μM Incubation time: For J774A.1: 6 h, 12 h; For THP-1: 1 hour; 2 h before 1 μg/mL LPS stimulated and another 30 min before 5 mM ATP induced Result: Led to a sharp decrease in NLRP3 expression in J774A.1 cells. Led to a large decrease in NLRP3 protein expression in a dose-dependent manner in THP-1 cells. Immunofluorescence Cell line: RAW264.7 macrophages, THP-1 cells Concentration: 20, 40, and 80 μM. Incubation time: Pre-treated for 1 h, continue to incubate for 18 h. Result: Decreased caspase-1 p20 activation and mature IL-1β and inhibited cleaved caspase-1 release into supernatants.

Storage & Handling

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Storage	Storage temperature: -20°C. Stability: ≥ 2 years
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

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Purity:	99.98%
CAS Number:	76588-87-9
MW:	574.57
Formula:	C₂₆H₃₈O₁₄

Yadanzigan

Description

Images & Validation

Key Properties

Bioactivity

Storage & Handling

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Yadanzigan (orb3054382)

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