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ZNF148 Antibody
SKU: orb750490
Description
Images & Validation
−Item 1 of 4
| Tested Applications | ELISA, WB |
|---|---|
| Dilution Range | ELISA: 1:10,000 - 1:30,000, WB: 1:5,000 |
| Reactivity | Human |
| Application Notes |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | Antiserum |
| Immunogen | Purified full length ZBP-89 recombinant protein expressed in E.coli. |
| Target | ZNF148 |
| Purity | Anti-ZBP89 antibody was prepared from monospecific antiserum by delipidation and defibrination. This polyclonal antibody is specific for human ZBP-89. Reactivity with ZBP-89 from other species has not been determined. |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Store ZBP89 Antibody at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. |
|---|---|
| Form/Appearance | Liquid (sterile filtered) |
| Buffer/Preservatives | 0.1% (w/v) Sodium Azide |
| Concentration | 90 mg/mL |
| Expiration Date | 12 months from date of receipt. |
| Dry Ice Shipping | Please note: This product requires shipment on dry ice. A dry ice surcharge will apply. |
| Disclaimer | For research use only |
Alternative Names
−rabbit anti-ZBP-89 Antibody, Transcription factor ZBP89 antibody, Zinc finger DNA binding protein 89 antibody, Zinc finger protein 148 antibody, ZNF 148 antibody, Zinc finger protein 148, Transcription factor ZBP-89, ZNF148
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Anti-ZBP-89 antibody used to confirm siRNA knockdown of ZBP-89.
Serum starvation induces ZBP-89 and p53 expression. AGS (gastric carcinoma) cells were cultured in serum-free F-12 medium for the indicated times, and western blots were used to detect the expression profiles of ZBP-89, p53, and p14ARF. Blotting was with Biorbyt's Rabbit-anti-ZBP-89 antibody. For detection use Biorbyt's HRP conjugated Gt-anti-Rabbit IgG MX10 (orb347654).
ZBP-89 interacts with p53 in vivo and in vitro. ZBP-89 interacts with amino acids 160 to 393 of p53. p53 contains an activation domain (AD), DNA binding domain (DNA BD), tetramerization domain (TD), and regulatory domain (RD). p53 binds to the zinc finger DNA binding domain of ZBP-89 (amino acids 154 to 300). ZBP-89 contains an acidic domain (AD), zinc finger DNA binding domain, basic domain (BD), and C-terminal domain (C-TER).
Quick Database Links
UniProt Details
− No UniProt data available
NCBI Reference Sequences
−Associated Accession Numbers
Curated reference sequences for the gene transcript and protein product| RefSeq | CAA15422.1 |
|---|
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Datasheet
Product Information
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Protocol Information
WB
Western Blot (IB, immunoblot)
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
ZNF148 Antibody (orb750490)
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