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Description
Research Area
Signal Transduction
Images & Validation
−
Item 1 of 7
| Tested Applications | ICC, IHC-Fr, IP, WB |
|---|---|
| Dilution Range | WB: 1:500-1:3000 , IF: 1:100-1:1000 , IHC-P: 1:100-1:1000 |
| Reactivity | Fish, Human, Mouse, Rat |
| Application Notes |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | Recombinant protein encompassing a sequence within the N-terminus region of human ZO-1. The exact sequence is proprietary. |
| Target | ZO1 |
| Molecular Weight | 195 |
| Purity | Purified by antigen-affinity chromatography. |
| Purification | Purified by antigen-affinity chromatography. |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°lC or below. Avoid multiple freeze-thaw cycles. |
|---|---|
| Form/Appearance | Liquid: 1XPBS, 1% rAlbumin, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative. |
| Buffer/Preservatives | PBS, 20% Glycerol, 0.025% ProClin 300. |
| Concentration | 0.4 mg/ml |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−tight junction protein 1 , ZO-1
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Quick Database Links
Gene Symbol
ZO1
UniProt
UniProt Details
− No UniProt data available
Protocol Information
WB
Western Blot (IB, immunoblot)
IHC-Fr
Immunohistochemistry Frozen
ICC
Immunocytochemistry
IP
Immunoprecipitation
Available Sizes
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