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Description
Research Area
Cancer Biology; Musculoskeletal & Connective Tissue Research
Images & Validation
−Item 1 of 6
| Tested Applications | IF, IHC, WB |
|---|---|
| Dilution Range | WB: 1:500:1000, IHC-P: 1:100:200 |
| Reactivity | Bovine, Canine, Gallus, Human, Monkey, Mouse, Porcine, Rabbit, Rat, Sheep |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Immunogen | KLH-conjugated synthetic peptide encompassing a sequence within the N-term region of human Beta-actin. The exact sequence is proprietary. |
| Target | ACTB |
| Molecular Weight | 41 kDa |
| Purification | The antibody was purified by immunogen affinity chromatography. |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Form/Appearance | Liquid |
| Buffer/Preservatives | 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−Actin cytoplasmic 1; Beta-actin
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Immunofluorescent analysis of Beta-actin staining in C6 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark. Phalloidin - AF594 was used to stain Actin filaments (red). DAPI was used to stain the cell nuclei (blue).
Western blot analysis of 293T (1) using Beta-actin Rabbit Polyclonal Antibody orb393034 with dilution at 1:1000.
Immunohistochemical analysis of paraffin-embedded human lung cancer using Beta-actin Rabbit Polyclonal Antibody orb393034 with dilution at 1:100.
Immunohistochemical analysis of paraffin-embedded rat kidney using Beta-actin Rabbit Polyclonal Antibody orb393034 with dilution at 1:100.
Immunohistochemical analysis of paraffin-embedded rat spleen using Beta-actin Rabbit Polyclonal Antibody orb393034 with dilution at 1:100.
Immunohistochemical analysis of paraffin-embedded rat stomach using Beta-actin Rabbit Polyclonal Antibody orb393034 with dilution at 1:100.
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC
Immunohistochemistry
IF
Immunofluorescence
Beta-actin Rabbit Polyclonal Antibody (orb393034)
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