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Beta-actin Rabbit Polyclonal Antibody
SKU: orb1294415
Description
Images & Validation
−Item 1 of 7
| Tested Applications | IF, IHC, WB |
|---|---|
| Dilution Range | Western Blot 1:2000-1:5000, Immunofluorescence 1:600-1:700, Immunohistochemistry (Paraffin) 1:400-1:600 |
| Reactivity | Human, Mouse, Rat |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | Rabbit-IgG |
| Immunogen | Synthetic peptide / encompassing a sequence within the C-terminus region. |
| Target | Beta-actin |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Buffer/Preservatives | 100mM Tris Glycine, 1% rAlbumin, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
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Anti-Beta Actin antibody - at 1/2000 dilution, Lysates at 40 µg per lane. This blot was produced using a 10% SDS-PAGE. Nitrocellulose membrane was then blocked for an hour before being incubated with orb1294415 overnight at 4°C.
Immunofluorescence: cells were fixed with 4% PFAfor 10 min at RT, permeabilized with 0.1% NP-40 for 10 min at RT then blocked with 5% BSA for 30 min at room temperature. Cells were stained with orb1294415 anti-Beta Actin antibody (red) at 1:400 and 4°C.
Immunofluorescence: cells were fixed with 4% PFAfor 10 min at RT, permeabilized with 0.1% NP-40 for 10 min at RT then blocked with 5% BSA for 30 min at room temperature. Cells were stained with orb1294415 anti-Beta Actin antibody (red) at 1:400 and 4°C.
Immunofluorescence: cells were fixed with 4% PFAfor 10 min at RT, permeabilized with 0.1% NP-40 for 10 min at RT then blocked with 5% BSA for 30 min at room temperature. Cells were stained with orb1294415 anti-Beta Actin antibody (red) at 1:400 and 4°C.
Immunofluorescence: cells were fixed with 4% PFAfor 10 min at RT, permeabilized with 0.1% NP-40 for 10 min at RT then blocked with 5% BSA for 30 min at room temperature. Cells were stained with orb1294415 anti-Beta Actin antibody (red) at 1:400 and 4°C.
Immunohistochemical analysis of paraffin embedded Human cancer tissue labeling Beta Actin with orb1294415 at 1/600.
Immunohistochemical analysis of paraffin embedded Human cancer tissue labeling Beta Actin with orb1294415 at 1/600.
Quick Database Links
Gene Symbol
Beta-actin
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Datasheet
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC
Immunohistochemistry
IF
Immunofluorescence
Beta-actin Rabbit Polyclonal Antibody (orb1294415)
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