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CD40 Antibody
SKU: orb1174068
Featured
Description
Images & Validation
−Item 1 of 5
| Tested Applications | ELISA, FC, IHC, WB |
|---|---|
| Dilution Range | ELISA 1/5000-10000|Flow Cyt 1/100 |
| Reactivity | Human |
Key Properties
−| Antibody Type | Recombinant Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Monoclonal |
| Isotype | Rabbit IgG |
| Clone No. | DM100 |
| Target | CD40 |
| Purification | Purified from cell culture supernatant by affinity chromatography |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Form/Appearance | Liquid |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−CD40; Bp50; CDW40; MGC9013; TNFRSF5; p50
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![Anti-CD40 [G28.5]](/images/pub/media/catalog/product/NewWebsite/35/orb348899_1.png)
![Anti-CD40 [G28.5]](/images/pub/media/catalog/product/NewWebsite/35/orb348899_2.png)
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ELISA plate pre-coated by 2 μg/ml (100 μL/well) Human CD40 protein, mFc-His tagged protein orb689376 can bind Rabbit anti-CD40 monoclonal antibody (clone: DM100) in a linear range of 0.64-80 ng/ml.
A. Flow cytometry analysis with Anti-CD40 (DM100) on Expi293 cells transfected with human CD40 (Blue histogram) or Expi293 transfected with irrelevant protein (Red histogram).
Flow cytometry analysis of antigen binding of rabbit anti-human CD40 mAb (orb1174068). (A) orb1174068 does not bind to Jurkat cells that do not express CD40. (B) A clear peak shift of orb1174068 was seen compared to the control when incubated with CD40-expressing Raji cells, indicating strong binding of orb1174068 to CD40. Antibodies were incubated at 2 μg/mL.
Anti-CD40 antibody (SKU# orb1174068) at 1/1000 dilution.Lane: Raji (human Burkitt's lymphoma B lymphocyte), whole cell lysate.Secondary: Goat Anti-Rabbit IgG H&L (HRP) at 1/5000 dilution.Predicted band size: 30 kDa.Observed band size: 45 kDa
A. orb1174068 at 10 μg/ml staining CD40 in Raji cells by IHC (SKU# orb1174068); B. orb1174068 at 10 μg/ml staining CD40 in human tonsil tissue by IHC (SKU# orb1174068)
Quick Database Links
Gene Symbol
CD40
UniProt
UniProt Details
− No UniProt data available
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Datasheet
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC
Immunohistochemistry
FC
Flow Cytometry
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
CD40 Antibody (orb1174068)
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