CD16 Antibody (FITC)

Reactivity of Anti-human CD16 FITC antibody (clone MEM-154) on human peripheral leukocytes Analysis of the antibody staining profile was performed on blood leukocytes isolated from peripheral whole blood by bulk erythrocyte lysis using 10× diluted EXCELLYSE Live. Suspension of blood leukocytes (2 x 10^6 cells) was added to the mixture of CD16 FITC antibody (clone MEM-154, 6.7 µg/ml in stained blood sample), backbone antibody conjugates and Monocyte Blocking Buffer, vortexed and incubated for 20 min. Stained sample was fixed with 2 ml of 10× diluted EXCELLYSE Easy solution for 10 min. Finally, samples were centrifuged (670 g, 5 min.), supernatant removed and the cell pellet was resuspended in 200 µl of PBS for acquisition. Donor of tested blood sample had 158F polymorphism on CD16a.

Anti-human CD16 FITC antibody (clone MEM-154) works in flow cytometry application. Analysis of the antibody staining profile was performed on blood leukocytes isolated from peripheral whole blood by bulk erythrocyte lysis using 10× diluted EXCELLYSE Live. Mouse monoclonal anti-human CD16 FITC antibody (clone MEM-154) was used in concentration 6.7 µg/ml in stained blood sample (2 x 10^6 cells). Donor of tested blood sample had 158F polymorphism on CD16a.