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Biotin

SKU: orb1309718

Description

Biotin

Research Area

Metabolism Research

Customer Validated Data

The data below is submitted by researchers worldwide. We provide this transparency to help you decide if this product works for your specific application or species, even if we haven't tested it ourselves.
Success by Application
Reactivity Distribution

Latest Experiments

1 Results
Dilution
Sample
Summary
RNA pulldown assay
Human
-
Cell lysate
It is used in the RNA...

Images & Validation

Key Properties

CAS Number58-85-5
MW244.31
Purity99.85%
FormulaC10H16N2O3S
SMILESC(CCCC(O)=O)[C@H]1[C@@]2([C@@](NC(=O)N2)(CS1)[H])[H]
TargetEndogenous Metabolite,Vitamin
SolubilityEthanol: < 1 mg/mL (insoluble or slightly soluble). DMSO: 130 mg/mL (532.11 mM), Sonication is recommended.

Bioactivity

In Vivo
Biotin serves as a coenzyme for carboxylases, involved in the synthesis of fatty acids, isoleucine, and valine, as well as in gluconeogenesis. In biological research, it is primarily used to label proteins for biochemical studies.
In Vitro
METHODS: To induce significant biotinylation of neighboring proteins, supplementation with Biotin is required at concentrations typically between 10-50 µM, with optimal incubation times between 15-18 h, after which total biotinylation plateaus. RESULTS: Due to the formation of covalent bonds between biotin and lysine residues on proteins, biotinylation appears to remain constant throughout the life cycle of the protein and can withstand stringent cleavage and washing conditions, thus maximizing the purity of isolated proteins identified by MS. The identified proteins can then be investigated as candidate interactors with POIs. METHODS: Mouse oligodendrocytes 158N were treated with Biotin (1-1000 nM) for 2 h, followed by the addition of 7β-OHC (50 µM) for a further 24 h. Cell viability was measured by MTT Assay. RESULTS: 7β-OHC-induced cytotoxicity was only prevented by Biotin at a concentration of 10-1000 nM.

Storage & Handling

Storagekeep away from direct sunlight | Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.
Expiration Date12 months from date of receipt.
DisclaimerFor research use only

Alternative Names

water-soluble B vitamin, Vitamin H, Vitamin B 7, Vitamin B7, Vitamin B-7, VB7, Endogenous Metabolite, essential nutrient, gluconeogenesis, Inhibitor, inhibit, 22879-79-4, Biotin, carboxylases, D-Biotin

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Quality Guarantee

Quality Guarantee

Explore bioreagents carefree to elevate your research. All our products are rigorously tested for performance. If a product does not perform as described on its datasheet, our scientific support team will provide expert troubleshooting, a prompt replacement, or a refund. For full details, please see our Terms & Conditions and Buying Guide. Contact us at support@biorbyt.com.

Key Properties

No computed properties available.

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Protocol Information

Biotin (orb1309718)

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% DMSO +
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Available Sizes

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200 mg
$ 80.00
1 ml x 10 mM (in DMSO)
$ 90.00
500 mg
$ 90.00
1 g
$ 120.00
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