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Biotin Antibody
SKU: orb1822658
Description
Images & Validation
−Item 1 of 2
| Tested Applications | FACS, IF, IHC-P, WB |
|---|---|
| Dilution Range | Flow cytometry: 0.5-1ug/million cells,Immunofluorescence: 0.5-1ug/ml,Western blot: 0.5-2ug/ml,Immunohistochemistry (FFPE): 0.5-1ug/ml for 30 minutes at RT |
| Reactivity | All |
| Application Notes |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Mouse |
| Clonality | Recombinant |
| Isotype | Mouse IgG1, kappa |
| Clone No. | rBTN/8819 |
| Immunogen | Biotinylated sheep immunoglobulin was used as the immunogen for the Biotin antibody. |
| Purification | Protein A/G affinity |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Buffer/Preservatives | 1 mg/ml in 1X PBS; rAlbumin free, sodium azide free |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
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IHC staining of FFPE human kidney tissue with biotinylated Lambda Light Chain antibody probe followed by Biotin antibody (clone rBTN/8819).
SDS-PAGE analysis of purified, BSA-free Biotin antibody (clone rBTN/8819) as confirmation of integrity and purity.
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC-P
Immunohistochemistry Paraffin
FACS
Fluorescence-Activated Cell Sorting (FC, Flow cytometry)
IF
Immunofluorescence
Biotin Antibody (orb1822658)
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